de Antonio Jesús Chacón, Fernández-Olmos Ana, Mercadillo María, Lindemann María Luisa Mateos, Mochales Fernando Baquero
Service of Microbiology, Ramón y Cajal University Hospital, Ctra. Colmenar, Km 9.1, 28034 Madrid, Spain.
J Virol Methods. 2008 Apr;149(1):163-6. doi: 10.1016/j.jviromet.2008.01.021. Epub 2008 Mar 6.
A number of human papillomaviruses (HPV) are the etiological agents of cervical cancer. The present study compared the performance of the hybrid capture method with that of linear array for the detection of high-risk HPV in 218 cervical samples. For the linear array technique, the DNA was extracted using two different procedures, one manual and the other automated. There was no difference in high-risk HPV (HR-HPV) detectability between the two extraction procedures but the automated procedure had the advantages of simplicity, time and efficiency. There was agreement in 199 (91.3%) of the results. The K value for the two assays was 0.81 indicative of "near perfect" agreement. Both methods, hybrid capture and linear array, are sensitive options for detection of HPV in cervical samples. Linear array enables the identification of the genotype present in the sample and the presence of multiple infections.
多种人乳头瘤病毒（HPV）是宫颈癌的病原体。本研究比较了杂交捕获法和线性阵列法在检测218份宫颈样本中高危HPV的性能。对于线性阵列技术，使用两种不同的程序提取DNA，一种是手动提取，另一种是自动提取。两种提取程序在高危HPV（HR-HPV）检测能力上没有差异，但自动提取程序具有操作简单、省时和高效的优点。199份（91.3%）结果一致。两种检测方法的K值为0.81，表明“近乎完美”的一致性。杂交捕获法和线性阵列法这两种方法都是检测宫颈样本中HPV的敏感方法。线性阵列法能够鉴定样本中存在的基因型以及多种感染的存在情况。
