Herbinière Juline, Grève Pierre, Strub Jean-Marc, Thiersé Danièle, Raimond Maryline, van Dorsselaer Alain, Martin Gilbert, Braquart-Varnier Christine
Laboratoire de Génétique et Biologie des Populations de Crustacés, Université de Poitiers, UMR CNRS 6556, Poitiers, France.
Dev Comp Immunol. 2008;32(8):875-82. doi: 10.1016/j.dci.2008.01.007. Epub 2008 Feb 14.
To establish and maintain a successful infection, microbial pathogens have evolved various strategies to infect the host in the face of a functional immune system. In this context, the alpha-proteobacteria Wolbachia capacities to infect new host species have been greatly evidenced. Indeed, in terrestrial isopods, experimentally transferred Wolbachia invade all host tissues, including immune cells such as hemocytes. To investigate mechanisms that have to be avoided by bacteria to maintain themselves in hemocytes, we characterized the hemocyte proteome of Armadillidium vulgare by a 2D gel electrophoresis approach. Fifty-six proteins were identified and classified into functional groups (stress and immunity, glucose metabolisms, cytoskeleton, others). We focused on immune response and cytoskeleton proteins often exploited by bacteria to invade their host. From the microsequences obtained by mass spectrometry, PCR primers were designed to amplify seven partial cDNAs encoding masquerade, alpha2-macroglobulin, transglutaminase, MnSOD, calreticulin, cyclophilin, and vinculin, confirming their expression in hemocytes.
为了建立并维持成功的感染，微生物病原体已经进化出各种策略，以便在宿主具有功能性免疫系统的情况下感染宿主。在这种情况下，α-变形菌沃尔巴克氏体感染新宿主物种的能力已得到充分证明。事实上，在陆生等足类动物中，实验性转移的沃尔巴克氏体能侵入包括血细胞等免疫细胞在内的所有宿主组织。为了研究细菌在血细胞中维持自身生存必须避免的机制，我们采用二维凝胶电泳方法对普通鼠妇的血细胞蛋白质组进行了表征。鉴定出56种蛋白质，并将其分为功能组（应激与免疫、葡萄糖代谢、细胞骨架等）。我们重点关注细菌经常利用来侵入宿主的免疫反应和细胞骨架蛋白。根据质谱获得的微序列，设计了PCR引物，以扩增七个部分cDNA，它们分别编码伪装蛋白、α2-巨球蛋白、转谷氨酰胺酶、锰超氧化物歧化酶、钙网蛋白、亲环蛋白和纽蛋白，证实了它们在血细胞中的表达。
