Rougé Carole, Des Robert Clotilde, Robins Alexander, Le Bacquer Olivier, De La Cochetière Marie-France, Darmaun Dominique
INRA, UMR Physiologie des Adaptations Nutritionnelles, University of Nantes, and CRNH (Human Nutrition Research Center), Nantes, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):40-7. doi: 10.1016/j.jchromb.2008.01.055. Epub 2008 Feb 20.
A method was developed by using gas chromatography-mass spectrometry in the electron impact ionization mode to quantify citrulline in plasma, red blood cells (RBC) and urine. For all three fluids, citrulline was extracted on ion exchange resins, before derivatization to its propyl-heptaflorobutyryl-ester. Assay precision (coefficient of variation, CV) was <5%, recovery% was >90% and the within- and between-day CV were <10% on 200 microL of plasma and RBC, and 400 microL of urine. The current method allows for the detection of 20 pmol of natural citrulline in aqueous standards, and small volumes (<100 microL) of biological fluids.
