Determination of citrulline in human plasma, red blood cells and urine by electron impact (EI) ionization gas chromatography-mass spectrometry.

作者信息

Rougé Carole, Des Robert Clotilde, Robins Alexander, Le Bacquer Olivier, De La Cochetière Marie-France, Darmaun Dominique

机构信息

INRA, UMR Physiologie des Adaptations Nutritionnelles, University of Nantes, and CRNH (Human Nutrition Research Center), Nantes, France.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):40-7. doi: 10.1016/j.jchromb.2008.01.055. Epub 2008 Feb 20.

DOI:10.1016/j.jchromb.2008.01.055

PMID:18329346

Abstract

A method was developed by using gas chromatography-mass spectrometry in the electron impact ionization mode to quantify citrulline in plasma, red blood cells (RBC) and urine. For all three fluids, citrulline was extracted on ion exchange resins, before derivatization to its propyl-heptaflorobutyryl-ester. Assay precision (coefficient of variation, CV) was <5%, recovery% was >90% and the within- and between-day CV were <10% on 200 microL of plasma and RBC, and 400 microL of urine. The current method allows for the detection of 20 pmol of natural citrulline in aqueous standards, and small volumes (<100 microL) of biological fluids.

摘要