Braakman E, Sturm E, Vijverberg K, van Krimpen B A, Gratama J W, Bolhuis R L
Department of Immunology, Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Int Immunol. 1991 Jul;3(7):691-7. doi: 10.1093/intimm/3.7.691.
Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, all V gamma 9-V delta 2 gamma delta T cells reportedly express CD45RO whereas all V delta gamma delta T cells lack CD45RO. Here, we show that these CD45RO- V delta gamma delta T cells all express CD45RA and the CD45RO+ V.9-V delta 2 gamma delta cells lack expression of CD45RA. The V delta T cells acquired CD45RO expression and lost part of their surface CD45RA, following in vitro activation with phytohaemagglutinin or IL-2. Also the CD3-CD16+ NK cells in peripheral blood that are uniformly CD45RA+ CD45RO- completely converted to the CD45RA-CD45RO+ phenotype upon in vitro activation. Moreover, all cloned V.9-V delta 2 and V delta 1 T cells and NK cells express CD45RO and lack expression of CD45RA. Our results strongly suggest that CD45RA and CD45RO are genuine markers for naive and primed lymphocytes that represent distinct differentiation lineages.
初始αβT细胞和致敏αβT细胞可以分别根据它们对CD45RA和CD45RO的差异表达来区分。本研究表明，这些CD45异构体也可识别γδT细胞和自然杀伤（NK）细胞的初始和致敏成熟阶段。据报道，在外周血中，所有Vγ9 - Vδ2γδT细胞均表达CD45RO，而所有VδγδT细胞均缺乏CD45RO。在此，我们表明这些CD45RO - VδγδT细胞均表达CD45RA，而CD45RO + Vγ9 - Vδ2γδ细胞则缺乏CD45RA的表达。在用植物血凝素或白细胞介素 - 2进行体外激活后，VδT细胞获得了CD45RO的表达并失去了其部分表面CD45RA。外周血中均为CD45RA + CD45RO - 的CD3 - CD16 + NK细胞在体外激活后也完全转变为CD45RA - CD45RO + 表型。此外，所有克隆的Vγ9 - Vδ2和Vδ1 T细胞以及NK细胞均表达CD45RO且缺乏CD45RA的表达。我们的结果强烈表明，CD45RA和CD45RO是代表不同分化谱系的初始和致敏淋巴细胞的真正标志物。
