He Zhishui, Xie Rong, Wang Yanzhang, Zou Huasong, Zhu Jiabi, Yu Guanqiao
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Acta Biochim Biophys Sin (Shanghai). 2008 Mar;40(3):209-16. doi: 10.1111/j.1745-7270.2008.00394.x.
Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in alfalfa nodule development.
热休克蛋白70（HSP70）家族的一些成员在生物体发育中具有重要功能。在此，我们利用cDNA阵列和逆转录-聚合酶链反应鉴定了一个苜蓿（Medicago sativa L. cv. Algonquin）HSP70基因，MsHSP70-1。该基因包含一个1947 bp的开放阅读框，编码一个649个氨基酸的蛋白质。该蛋白质包含植物HSP70s的所有保守结构域、基序和特征序列。与根、茎、叶和花相比，MsHSP70-1在根瘤中的表达增强，并且在根瘤发育的整个过程中均如此。Northern杂交分析表明，MsHSP70-1在根瘤中的表达需要根瘤菌的活性bacA基因。这些结果表明，MsHSP70-1可能在苜蓿根瘤发育中起重要作用。
