表观遗传学：通过限制性内切酶标记基因组扫描对胚胎干细胞的研究。
Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning.
作者信息
Hattori Naka, Shiota Kunio
机构信息
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, University of Tokyo, Japan.
出版信息
FEBS J. 2008 Apr;275(8):1624-30. doi: 10.1111/j.1742-4658.2008.06331.x. Epub 2008 Mar 7.
During mammalian development, it is essential that the proper epigenetic state is established across the entire genome in each differentiated cell. To date, little is known about the mechanism for establishing epigenetic modifications of individual genes during the course of cellular differentiation. Genome-wide DNA methylation analysis of embryonic stem cells by restriction landmark genomic scanning provides information about cell type- and tissue-specific DNA methylation profiles at tissue-specific methylated regions associated with developmental processes. It also sheds light on DNA methylation alterations following fetal exposure to chemical agents. In addition, analysis of embryonic stem cells deficient in epigenetic regulators will contribute to revealing the mechanism for establishing DNA methylation profiles and the interplay between DNA methylation and other epigenetic modifications.
在哺乳动物发育过程中，每个分化细胞的整个基因组都建立起合适的表观遗传状态至关重要。迄今为止，关于细胞分化过程中单个基因表观遗传修饰的建立机制知之甚少。通过限制性地标基因组扫描对胚胎干细胞进行全基因组DNA甲基化分析，可提供与发育过程相关的组织特异性甲基化区域的细胞类型和组织特异性DNA甲基化图谱信息。它还能揭示胎儿接触化学物质后DNA甲基化的变化。此外，对表观遗传调节因子缺陷的胚胎干细胞进行分析，将有助于揭示DNA甲基化图谱的建立机制以及DNA甲基化与其他表观遗传修饰之间的相互作用。
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