Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul 153-803, Korea.
BMB Rep. 2010 Dec;43(12):830-5. doi: 10.5483/BMBRep.2010.43.12.830.
Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
通过 DNA 甲基化对基因组进行表观遗传修饰是维持人类胚胎干细胞（hESC）分化状态的关键，并且在分化过程中必须通过视黄酸（RA）处理来重置。为了鉴定 RA 处理的 hESC 的表观遗传修饰，进行了全基因组甲基化/基因表达分析。在未分化和 RA 处理的 hESC 之间，发现了 166 个差异甲基化 CpG 位点和 2013 个差异表达基因。甲基化和表达数据分析的综合分析表明，有 19 个基因（STAP2、VAMP8、C10orf26、WFIKKN1、ELF3、C1QTNF6、C10orf10、MRGPRF、ARSE、LSAMP、CENTD3、LDB2、POU5F1、GSPT2、THY1、ZNF574、MSX1、SCMH1 和 RARB）彼此之间高度相关。本研究提供的结果将通过进一步的功能和生物学研究，促进对 DNA 甲基化和基因表达之间相互作用的未来研究。
