Ichinohe Norihisa, Takamoto Tomoaki, Tabata Yasuhiko
Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University, Sapporo, Japan.
Tissue Eng Part A. 2008 Jan;14(1):107-16. doi: 10.1089/ten.a.2007.0021.
The proliferation, osteogenic differentiation, and distribution patterns of stromal cells from rat bone marrow were investigated in a three-dimensional nonwoven fabric of polyethylene terephthalate fiber by the static, agitated, and stirred culture methods; stirring speeds were 10, 50, and 100 rpm in the stirred culture method. The culture method affected the time profile of proliferation and osteogenic differentiation of cells or their distribution in the fabric. The extent of cell proliferation and osteogenic differentiation became higher in order of the stirred at 100 rpm = the stirred at 50 rpm > the stirred at 10 rpm > the agitated > the static methods. In addition, the cells were more uniformly proliferated in the fabric by the stirred culture method with time than they were proliferated in the fabric by other methods. The alkaline phosphatase (ALP) activity and calcium content were higher for cells cultured by the stirred culture method than those cultured by other methods. The total ALP activity, calcium content, and bone mineral density were higher for every stirred method than those for other methods. However, the distribution uniformity of cells differentiated was low irrespective of the culture method. It is concluded that the extent of proliferation and differentiation of cells or their distribution uniformity in the nonwoven fabrics was influenced by the culture method.
采用静态、振荡和搅拌培养方法，在聚对苯二甲酸乙二酯纤维三维无纺布中研究大鼠骨髓基质细胞的增殖、成骨分化及分布模式；搅拌培养方法中的搅拌速度分别为10、50和100 rpm。培养方法影响细胞增殖和成骨分化的时间进程及其在织物中的分布。细胞增殖和成骨分化程度按以下顺序升高：100 rpm搅拌培养 = 50 rpm搅拌培养 > 10 rpm搅拌培养 > 振荡培养 > 静态培养。此外，随着时间推移，搅拌培养方法使细胞在织物中比其他方法更均匀地增殖。搅拌培养法培养的细胞碱性磷酸酶（ALP）活性和钙含量高于其他方法培养的细胞。每种搅拌培养法的总ALP活性、钙含量和骨矿物质密度均高于其他方法。然而，无论采用何种培养方法，分化细胞的分布均匀性都较低。得出结论：细胞在无纺布中的增殖和分化程度或其分布均匀性受培养方法影响。
