Desai N P, Hubbell J A
Department of Chemical Engineering, University of Texas, Austin 78712.
J Biomed Mater Res. 1991 Jul;25(7):829-43. doi: 10.1002/jbm.820250704.
Polyethylene oxide (PEO) of molecular weights 5,000, 10,000, 18,500, and 100,000 g/mol was covalently grafted to surfaces of otherwise cell adhesive polyethylene terephthalate (PET) films. Analysis of these surfaces by measurement of contact angles and ESCA verified the presence of the grafted PEO. Protein adsorption assays of radiolabeled albumin and fibrinogen showed a marked reduction in adsorbed protein for the 18,500 and 100,000 molecular weight PEO coupled surfaces. Cell growth assays using human foreskin fibroblasts in culture showed that the higher-molecular-weight PEO surfaces supported cell growth to a much lower extent than the two lower-molecular-weight PEOs. Flow of whole blood over these surfaces and visualization of platelet adherence using epifluorescence video-microscopy showed very low platelet adherence only on the two higher-molecular-weight PEO coupled surfaces. Scanning electron microscopy corroborated these results. It was concluded that PEO of molecular weights neighboring 18,500 and higher was effective in reducing protein adsorption and cellular interactions on these surfaces.
将分子量分别为5000、10000、18500和100000 g/mol的聚环氧乙烷（PEO）共价接枝到原本具有细胞粘附性的聚对苯二甲酸乙二酯（PET）薄膜表面。通过测量接触角和进行电子能谱化学分析（ESCA）对这些表面进行分析，证实了接枝的PEO的存在。对放射性标记的白蛋白和纤维蛋白原进行的蛋白质吸附试验表明，对于与分子量为18500和100000的PEO偶联的表面，吸附的蛋白质显著减少。使用培养的人包皮成纤维细胞进行的细胞生长试验表明，较高分子量的PEO表面支持细胞生长的程度远低于两种较低分子量的PEO。让全血在这些表面流动，并使用落射荧光视频显微镜观察血小板粘附情况，结果显示仅在与两种较高分子量的PEO偶联的表面上血小板粘附非常少。扫描电子显微镜证实了这些结果。得出的结论是，分子量接近18500及更高的PEO在减少这些表面上的蛋白质吸附和细胞相互作用方面是有效的。
