Benguedouar Lamia, Boussenane Hanane Nadia, Wided Kebsa, Alyane Mohamed, Rouibah Hassiba, Lahouel Mesbah
Laboratoire de Pharmacologie et Phytochimie, Faculté des Sciences, Université de Jijel. 18000 Jijel, Algérie.
Indian J Exp Biol. 2008 Feb;46(2):112-9.
To assess the oxidative stress and mitochondrial dysfunction associated with disease, toxic process and aging, in vivo and in vitro preventive effect of propolis extract against mitochondrial oxidative stress induced by two anticancer drugs (doxorubicin and vinblastin) have been investigated in female wistar rat using liver and heart mitochondria. The results show that doxorubicin and vinblastin altered mitochondrial functions as observed by a decrease in respiratory control value, an activation of swelling and overproduction of superoxide anion. Myocardial tissue from doxorubicin treated rats showed a marked increase in malondialdehyde production, a depletion of reduced glutathione contents and an inhibition of catalase and superoxide dismutase activities. Similar results were also observed in liver tissue. Pretreatment of rats with propolis extract (100 mg/kg/day po) (10(-4) M ip) administered 4 days prior to doxorubicin (20 mg/kg) and/or vinblastin (2 mg/kg) injection, substantially reduced the peroxidative damage in myocardium and hepatic tissues and markedly restored the tissues catalase and SOD activities. The results strongly suggest that propolis extract protects heart and liver tissues from oxidative stress by protecting the mitochondria.
为评估与疾病、毒性过程和衰老相关的氧化应激和线粒体功能障碍，利用雌性Wistar大鼠的肝脏和心脏线粒体，研究了蜂胶提取物对两种抗癌药物（阿霉素和长春碱）诱导的线粒体氧化应激的体内和体外预防作用。结果表明，阿霉素和长春碱改变了线粒体功能，表现为呼吸控制值降低、肿胀激活和超氧阴离子过量产生。阿霉素处理的大鼠心肌组织中丙二醛生成显著增加，还原型谷胱甘肽含量减少，过氧化氢酶和超氧化物歧化酶活性受到抑制。肝脏组织中也观察到类似结果。在注射阿霉素（20mg/kg）和/或长春碱（2mg/kg）前4天，给大鼠预先口服蜂胶提取物（100mg/kg/天）（腹腔注射10(-4)M），可显著降低心肌和肝组织中的过氧化损伤，并显著恢复组织中的过氧化氢酶和超氧化物歧化酶活性。结果强烈表明，蜂胶提取物通过保护线粒体来保护心脏和肝脏组织免受氧化应激。
