由CD45异构体定义的离散亚群共存于B细胞慢性淋巴细胞白血病患者的白血病细胞中。

Discrete subpopulations, defined by CD45 isoforms, coexist within the leukaemic cells of B-chronic lymphocytic leukaemia patients.

作者信息

Maddy A, Lyons A B, Wu Z W, Taylor H, Sanderson A, Mackie M

机构信息

Division of Biological Sciences, University of Edinburgh, U.K.

出版信息

Leuk Res. 1991;15(9):791-9. doi: 10.1016/0145-2126(91)90463-4.

DOI:10.1016/0145-2126(91)90463-4

PMID:1833598

Abstract

The expression of CD45 isoforms by B-CLL leukaemic lymphocytes has been analysed by 2 colour flow cytometry and vectorial iodination. The cytometry has demonstrated the presence of cells with different CD45 phenotypes which vary in the relative expression of the CD45RA and CD45RO determinants. Discrete populations have been detected which can coexist within an individual patient. One of these populations which is CD45RO-negative consists of cells expressing only the 230 kD isoform, in the others the smaller isoforms are expressed, the appearance of the CD45RO determinant of 180 kD being accompanied by the appearance of the 190 kD isoform. The relative proportion of cell populations is stable within patients but can be altered by phorbol ester which enhances CD45RO expression and diminishes CD45RA expression. The populations may be partially resolved by the density gradient fractionation.

摘要

通过双色流式细胞术和向量碘化分析了B - CLL白血病淋巴细胞中CD45亚型的表达。流式细胞术已证明存在具有不同CD45表型的细胞，这些细胞在CD45RA和CD45RO决定簇的相对表达上有所不同。已检测到离散的细胞群体，它们可在个体患者体内共存。其中一个CD45RO阴性群体由仅表达230 kD亚型的细胞组成，在其他群体中表达较小的亚型，180 kD的CD45RO决定簇的出现伴随着190 kD亚型的出现。细胞群体的相对比例在患者体内是稳定的，但可被佛波酯改变，佛波酯可增强CD45RO表达并减少CD45RA表达。这些群体可通过密度梯度分级分离得到部分分离。