van Heusden G Paul H, Steensma H Yde
Section Molecular and Developmental Genetics, Institute of Biology, Leiden University, AL Leiden, The Netherlands.
FEMS Microbiol Lett. 2008 May;282(1):100-4. doi: 10.1111/j.1574-6968.2008.01113.x. Epub 2008 Mar 10.
The Saccharomyces cerevisiae WSS1 (Weak Suppressor of Smt3) gene has initially been identified as a multicopy suppressor of a mutation in SMT3 encoding the small ubiquitin-like modifier. Later, multiple functions related to DNA replication and repair have been found for WSS1. Here, we report the subcellular location of the Wss1 protein. Fluorescence microscopy of strains expressing a Wss1p-green fluorescent protein (GFP) fusion shows that the protein is present in a single sharp spot near the nuclear membrane, distinct from the spindle pole bodies and nucleolus. In dividing cells, the spot is exclusively present in the mother cell, suggesting a mother cell-specific function of WSS1.
酿酒酵母WSS1（Smt3的弱抑制因子）基因最初被鉴定为编码小泛素样修饰物的SMT3基因突变的多拷贝抑制因子。后来，发现WSS1具有与DNA复制和修复相关的多种功能。在此，我们报告了Wss1蛋白的亚细胞定位。对表达Wss1p-绿色荧光蛋白（GFP）融合蛋白的菌株进行荧光显微镜观察，结果显示该蛋白存在于核膜附近的单个清晰斑点中，与纺锤极体和核仁不同。在分裂细胞中，该斑点仅存在于母细胞中，这表明WSS1具有母细胞特异性功能。
