Bousema J Teun, Drakeley Chris J, Mens Petra F, Arens Theo, Houben Rein, Omar Sabah A, Gouagna Louis C, Schallig Henk, Sauerwein Robert W
Department of Medical Microbiology, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands.
Am J Trop Med Hyg. 2008 Mar;78(3):442-8.
Plasmodium falciparum and P. malariae occur endemically in many parts of Africa. Observations from malariotherapy patients suggest that co-infection with P. malariae may increase P. falciparum gametocyte production. We determined P. falciparum gametocyte prevalence and density by quantitative nucleic acid sequence-based amplification (QT-NASBA) after antimalarial treatment of Kenyan children with either P. falciparum mono-infection or P. falciparum and P. malariae mixed infection. In addition, we analyzed the relationship between mixed species infections and microscopic P. falciparum gametocyte prevalence in three datasets from previously published studies. In Kenyan children, QT-NASBA gametocyte density was increased in mixed species infections (P = 0.03). We also observed higher microscopic prevalences of P. falciparum gametocytes in mixed species infections in studies from Tanzania and Kenya (odds ratio = 2.15, 95% confidence interval = 0.99-4.65 and 2.39, 1.58-3.63) but not in a study from Nigeria. These data suggest that co-infection with P. malariae is correlated with increased P. falciparum gametocytemia.
恶性疟原虫和三日疟原虫在非洲许多地区呈地方性流行。对疟疾病人进行疟原虫疗法的观察表明，同时感染三日疟原虫可能会增加恶性疟原虫配子体的产生。我们通过基于核酸序列的定量扩增（QT-NASBA）方法，测定了肯尼亚儿童在接受抗疟治疗后，单独感染恶性疟原虫或同时感染恶性疟原虫和三日疟原虫时，恶性疟原虫配子体的流行率和密度。此外，我们还分析了在先前发表的三项研究数据集中，混合感染与镜检恶性疟原虫配子体流行率之间的关系。在肯尼亚儿童中，混合感染时QT-NASBA检测到的配子体密度增加（P = 0.03）。我们还观察到，在坦桑尼亚和肯尼亚的研究中，混合感染时镜检恶性疟原虫配子体的流行率更高（优势比分别为2.15，95%置信区间为0.99 - 4.65；2.39，1.58 - 3.63），但在尼日利亚的一项研究中未观察到这种情况。这些数据表明，同时感染三日疟原虫与恶性疟原虫配子血症增加相关。
