Moss P A, Moots R J, Rosenberg W M, Rowland-Jones S J, Bodmer H C, McMichael A J, Bell J I
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8987-90. doi: 10.1073/pnas.88.20.8987.
The major histocompatibility complex class I molecule HLA-A2.1 presents the influenza A virus matrix peptide 57-68 to cytotoxic T lymphocytes in all individuals with this common HLA type and is among the most thoroughly studied immune responses in humans. We have studied the T-cell receptor (TCR) heterogeneity of T cells specific for HLA-A2 and influenza A matrix peptide using the polymerase chain reaction. The usage of V alpha and V beta sequences seen on these T cells is remarkably conserved as are certain junctional sequences associated with alpha and beta chains. Furthermore, two unrelated HLA-A2 individuals have a similar pattern of TCR usage, implying that this is a predominant response in HLA-A2 populations. Analysis in one individual showed that the conserved TCR V alpha and V beta genes are minor members of the peripheral blood TCR repertoire. The sequences provide important information on the TCR necessary for the final structural analysis of this ternary complex.
主要组织相容性复合体I类分子HLA - A2.1在所有具有这种常见HLA类型的个体中，将甲型流感病毒基质肽57 - 68呈递给细胞毒性T淋巴细胞，并且是人类中研究最为深入的免疫反应之一。我们使用聚合酶链反应研究了针对HLA - A2和甲型流感病毒基质肽的T细胞的T细胞受体（TCR）异质性。这些T细胞上可见的Vα和Vβ序列的使用情况以及与α和β链相关的某些连接序列都非常保守。此外，两名无关的HLA - A2个体具有相似的TCR使用模式，这意味着这是HLA - A2群体中的主要反应。对一名个体的分析表明，保守的TCR Vα和Vβ基因是外周血TCR库中的少数成员。这些序列为该三元复合物的最终结构分析所需的TCR提供了重要信息。
