Belton Michelle, Commerford Kristy, Hall Justin, Prato Frank S, Carson Jeffrey J L
Imaging Program, Lawson Health Research Institute, London, Ontario, Canada.
Bioelectromagnetics. 2008 Sep;29(6):439-46. doi: 10.1002/bem.20409.
Calcium ions are involved in a number of important signal transduction pathways in cells. Cytosolic calcium concentration (Ca(2+)) can be affected by the activation of Ca(2+) channels through the action of ligands such as ATP. The response of Ca(2+) to ligands may be affected by external factors like magnetic fields. The purpose of this study was to determine if exposure to a static magnetic field (SMF) for 800 s altered the Ca(2+) response to ATP in undifferentiated HL-60 cells. We sham exposed or field exposed fura-2 loaded HL-60 cells to a SMF of 1, 10, and 100 mT. Cells were activated with ATP 300 s into the exposure. The level of Ca(2+) was followed before, during, and after field or sham exposure with a ratiometric fluorescence spectroscopy system. It was found that high concentrations of ATP resulted in greater Ca(2+) responses, but faster recovery to near basal levels. The application of 1, 10, or 100 mT SMF did not affect the Ca(2+) response to ATP. Future work could examine the effect of a longer SMF exposure on the Ca(2+) response to ATP. Longer exposures might provide sufficient time for morphological changes in the plasma membrane to occur.
钙离子参与细胞内许多重要的信号转导途径。胞质钙浓度（Ca(2+)）可受ATP等配体作用激活Ca(2+)通道的影响。Ca(2+)对配体的反应可能受磁场等外部因素影响。本研究的目的是确定800秒的静磁场（SMF）暴露是否会改变未分化HL-60细胞中Ca(2+)对ATP的反应。我们将装载fura-2的HL-60细胞假暴露或磁场暴露于1、10和100 mT的静磁场中。在暴露300秒时用ATP激活细胞。用比率荧光光谱系统在磁场或假暴露之前、期间和之后跟踪Ca(2+)水平。发现高浓度ATP导致更大的Ca(2+)反应，但恢复到接近基础水平的速度更快。施加1、10或100 mT的静磁场不影响Ca(2+)对ATP的反应。未来的工作可以研究更长时间的静磁场暴露对Ca(2+)对ATP反应的影响。更长时间的暴露可能为质膜发生形态变化提供足够的时间。
