Matthews J B, Deacon E M, Kitas G D, Salmon M, Potts A J, Hamburger J, Bacon P A
Department of Oral Surgery, University of Birmingham, UK.
Virchows Arch A Pathol Anat Histopathol. 1991;419(3):191-7. doi: 10.1007/BF01626347.
This study has investigated the presence and distribution of B cells, T cells and T-cell subsets within labial glands of patients with primary Sjogren's syndrome (n = 9) and secondary Sjogren's syndrome associated with rheumatoid arthritis (n = 8) using a sequential double immunoperoxidase technique and true colour image analysis. The composition of the inflammatory infiltrates was similar in glands from both patient groups. B cells were normally present within large foci with few detected in diffuse infiltrates such that the ratio of T:B cells in foci (2.4:1) was significantly lower than in diffuse infiltrates (7.3:1; P less than 0.001). In all infiltrates helper T cells (CD8-, CD3+) predominated over suppressor/cytotoxic cells (CD8+, CD3+; 2.7:1). Analysis of primed (CD45RA-, CD45RO+) and naive (CD45RA+, CD45RO-) CD8- T cells showed that the ratio of the primed to naive subset was significantly higher in focal (4.2:1) compared to diffuse (1.5:1; P less than 0.001) areas of lymphoid infiltration. These results indicate that the focal lymphocytic infiltrates characteristic of Sjogren's syndrome contain B cells associated with a T-cell population consisting predominantly of primed CD8- helper T cells. This latter population may be responsible for upregulating glandular B-cell activity in Sjogren's syndrome.
本研究采用连续双重免疫过氧化物酶技术和真彩色图像分析，调查了原发性干燥综合征患者（n = 9）和类风湿关节炎相关继发性干燥综合征患者（n = 8）唇腺内B细胞、T细胞及T细胞亚群的存在情况和分布。两组患者腺体中炎性浸润的组成相似。B细胞通常存在于大的病灶内，在弥漫性浸润中检测到的较少，因此病灶中T:B细胞的比例（2.4:1）显著低于弥漫性浸润中的比例（7.3:1；P<0.001）。在所有浸润中，辅助性T细胞（CD8-，CD3+）多于抑制性/细胞毒性细胞（CD8+，CD3+；2.7:1）。对致敏（CD45RA-，CD45RO+）和未致敏（CD45RA+，CD45RO-）CD8-T细胞的分析表明，在淋巴浸润的病灶区域（4.2:1），致敏亚群与未致敏亚群的比例显著高于弥漫区域（1.5:1；P<0.001）。这些结果表明，干燥综合征特征性的灶性淋巴细胞浸润包含与主要由致敏CD8-辅助性T细胞组成的T细胞群体相关的B细胞。后一群体可能负责上调干燥综合征中腺B细胞的活性。
