Zhou Simon Ningsun, Ouyang Gangfeng, Pawliszyn Janusz
Department of Chemistry, University of Waterloo, Waterloo, Ont, Canada.
J Chromatogr A. 2008 Jul 4;1196-1197:46-56. doi: 10.1016/j.chroma.2008.02.068. Epub 2008 Feb 26.
Microdialysis (MD) and solid-phase microextraction (SPME) methods have been developed and compared through in vitro and in vivo studies. For in vitro study, both methods offered accurate and precise results for complex sample matrices by standard addition method. Compared to MD, the fully automated SPME procedure offered several advantages including high-throughput and more efficient sampling, less labor intensity, and capability for batch analysis. For in vivo study, although both techniques provided sampling with minimal perturbation to the system under study, SPME was more sensitive, precise and accurate, suitable for field sampling and had a wider application than MD. It demonstrated that SPME has the potential to replace MD for in vivo study.
微透析（MD）和固相微萃取（SPME）方法已经通过体外和体内研究得以开发和比较。对于体外研究，两种方法通过标准加入法对复杂样品基质都能提供准确且精密的结果。与微透析相比，全自动固相微萃取程序具有若干优势，包括高通量和更高效的采样、更低的劳动强度以及批量分析能力。对于体内研究，尽管两种技术都能在对所研究系统扰动最小的情况下进行采样，但固相微萃取更灵敏、精密且准确，适用于现场采样，并且比微透析有更广泛的应用。这表明固相微萃取在体内研究方面有取代微透析的潜力。
