染色质状态图谱：新技术，新见解。
Chromatin state maps: new technologies, new insights.
作者信息
Mendenhall Eric M, Bernstein Bradley E
机构信息
Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA.
出版信息
Curr Opin Genet Dev. 2008 Apr;18(2):109-15. doi: 10.1016/j.gde.2008.01.010. Epub 2008 Mar 12.
Recent years have seen unprecedented characterization of mammalian chromatin thanks to advances in chromatin assays, antibody development, and genomics. Genome-wide maps of chromatin state can now be readily acquired using microarrays or next-generation sequencing technologies. These datasets reveal local and long-range chromatin patterns that offer insight into the locations and functions of underlying regulatory elements and genes. These patterns are dynamic across developmental stages and lineages. Global studies of chromatin in embryonic stem cells have led to intriguing hypotheses regarding Polycomb/trithorax and RNA polymerase roles in 'poising' transcription. Chromatin state maps thus provide a rich resource for understanding chromatin at a 'systems level', and a starting point for mechanistic studies aimed at defining epigenetic controls that underlie development.
近年来，由于染色质分析、抗体研发和基因组学的进展，哺乳动物染色质得到了前所未有的表征。现在可以使用微阵列或下一代测序技术轻松获取全基因组染色质状态图谱。这些数据集揭示了局部和远距离的染色质模式，有助于深入了解潜在调控元件和基因的位置及功能。这些模式在发育阶段和细胞谱系中是动态变化的。对胚胎干细胞中染色质的全局性研究引发了关于多梳蛋白/三胸节蛋白以及RNA聚合酶在“准备”转录过程中作用的有趣假说。因此，染色质状态图谱为从“系统层面”理解染色质提供了丰富资源，也是旨在确定发育背后表观遗传控制机制的研究起点。
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