Martinho Filipa, Prieto Emília, Pinto Dulce, Castro Rita Maria, Morais Ana Maria, Salgado Luísa, Exposto Filomena da Luz
Sexually Transmitted Diseases Unit, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal.
Enferm Infecc Microbiol Clin. 2008 Feb;26(2):69-71. doi: 10.1157/13115539.
Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy.
Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained from 300 women were inoculated onto LB or ILB, or onto Todd-Hewitt broth and analyzed with the ATB system (comparison method).
Prevalence of GBS was 20% (61/300). LB and Todd-Hewitt with ATB detected GBS in 20% of women, and ILB in 19% of women. No growth was observed at four hours in any of the media studied. At 10 h and 14 h, identification of GBS was possible in 43/300 (14%) and 53/300 (18%) of ILB cultures, respectively, and in 32/300 (11%) and 46/300 (15%) of LB cultures.
All the media used are suitable for GBS detection. The majority of GBS were identified in ILB and LB cultures at 10 h and 14 h.
B族链球菌（GBS）可由母亲传播给新生儿。对妊娠35至37周时携带GBS的母亲进行产时预防可预防新生儿感染。
对用于GBS检测的液体双相格拉纳达培养基（LB）和即时液体双相格拉纳达培养基（ILB）进行评估。从300名女性中获取阴道拭子，接种到LB或ILB上，或接种到托德-休伊特肉汤上，并用ATB系统进行分析（比较方法）。
GBS的患病率为20%（61/300）。LB和托德-休伊特肉汤联合ATB系统在20%的女性中检测到GBS，ILB在19%的女性中检测到GBS。在所研究的任何培养基中，4小时时均未观察到生长。在10小时和14小时时，分别在43/300（14%）和53/300（18%）的ILB培养物中以及32/300（11%）和46/300（15%）的LB培养物中能够鉴定出GBS。
所有使用的培养基均适用于GBS检测。大多数GBS在10小时和14小时时在ILB和LB培养物中被鉴定出来。
Zotero 插件