Kaji T, Sakamoto M
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Blood Coagul Fibrinolysis. 1991 Jun;2(3):419-23. doi: 10.1097/00001721-199106000-00003.
We investigated the effect of plasmin on the release of glycosaminoglycans (GAG) from bovine aortic endothelial cells in culture. Plasmin significantly increased the release of [35S]sulphate-labelled GAG (35S-GAG) from the cell layer, although plasminogen did not show such a stimulatory effect. The percentage of heparan sulphate in the released 35S-GAG (about 80%) was not changed by plasmin. Plasmin enhanced the detachment of endothelial cells from the monolayer only slightly. From these results, it was suggested that the local anticoagulant activity on endothelium mediated by the anticoagulant heparan sulphate will be increased by plasmin; the conversion of plasminogen to plasmin may be mediated by plasminogen activators released from thrombin-stimulated endothelial cells.
我们研究了纤溶酶对培养的牛主动脉内皮细胞释放糖胺聚糖（GAG）的影响。纤溶酶显著增加了细胞层中[35S]硫酸盐标记的GAG（35S-GAG）的释放，而纤溶酶原未显示出这种刺激作用。纤溶酶不会改变释放的35S-GAG中硫酸乙酰肝素的百分比（约80%）。纤溶酶仅轻微增强了内皮细胞从单层的脱离。从这些结果表明，纤溶酶会增加由抗凝血硫酸乙酰肝素介导的内皮局部抗凝血活性；纤溶酶原向纤溶酶的转化可能由凝血酶刺激的内皮细胞释放的纤溶酶原激活剂介导。
