Wang You-Wun, Chern Lih-Ling, Cam Phung Dac, Chiou Chien-Shun
The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City 40855, Taiwan.
Diagn Microbiol Infect Dis. 2008 Jul;61(3):251-5. doi: 10.1016/j.diagmicrobio.2008.01.024. Epub 2008 Mar 14.
We evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus.
我们评估了用于副溶血性弧菌RIMD 2210633基因组脉冲场凝胶电泳（PFGE）分析的具有稀有切割位点的限制酶的成本效益。评估表明，使用NotI和SfiI进行PFGE具有区分性，但NotI更具成本效益。基于本研究结果，我们建议将NotI和SfiI分别作为第一和第二限制酶，用于标准化副溶血性弧菌分子分型和全球监测的PulseNet PFGE方案。
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