[采用脉冲场凝胶电泳和自动核糖体分型法对用MPN法分离的副溶血性弧菌进行同源性分析]
[Homology analysis of Vibrio parahaemolyticus isolated by MPN method using pulsed-field gel electrophoresis and automated ribotyping].
作者信息
Li Weiwei, Wang Xiaoying, Pei Xiaoyan, Zhu Haiming, Guo Yunchang
机构信息
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
出版信息
Wei Sheng Yan Jiu. 2010 May;39(3):374-80.
OBJECTIVE
To study the molecular typing of 78 strains of Vibrio parahaemolyticus isolated from Guangdong by MPN method with pulsed-field gel electrophoresis (PFGE) and automated ribotyping (RP).
METHODS
DNAs from all strains were digested by EcoR I for ribotyping and digested by Sfi I and Not I for PFGE. The band patterns of PFGE and RP were clustering adn analyzed by BioNumerics software.
RESULTS
There were 64, 68 and 71 kinds of band patterns of each Vibrio parahaemolyticus strain digested by restriction endonuclease EcoR I, Sfi I and Not I respectively. The band patterns of some strains from the same sample are completely identical, but the band patterns of most strains are different and are divided into different groups.
CONCLUSION
PFGE is a rapid, robust, and reproducible genotyping technique, more discriminative than automated ribotyping. Choosing more isolates from MPN method for suspected food samples should be considered in tracing the phylogenetic relationship of strains.
目的
采用脉冲场凝胶电泳（PFGE）和自动核糖体分型（RP）技术，通过MPN法对从广东分离的78株副溶血性弧菌进行分子分型研究。
方法
所有菌株的DNA用EcoR I酶切进行核糖体分型，用Sfi I和Not I酶切进行PFGE。PFGE和RP的条带模式通过BioNumerics软件进行聚类和分析。
结果
分别用限制性内切酶EcoR I、Sfi I和Not I酶切后，每株副溶血性弧菌的条带模式分别有64、68和71种。同一标本中部分菌株的条带模式完全相同，但大多数菌株的条带模式不同，被分为不同的组。
结论
PFGE是一种快速、可靠且可重复的基因分型技术，比自动核糖体分型更具鉴别力。在追踪菌株的系统发育关系时，应考虑从MPN法检测的可疑食品样本中选取更多分离株。
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