Jucker M, Kleinman H K, Höhmann C F, Ordy J M, Ingram D K
Laboratory of Cellular and Molecular Biology, Nathan W. Shock Laboratories, National Institute on Aging, NIH, Baltimore, MD 21224.
Brain Res. 1991 Aug 2;555(2):305-12. doi: 10.1016/0006-8993(91)90356-z.
A phosphorylated, approximately 110 kDa laminin-binding protein (110 kDa LBP) from mouse brain has been previously identified. This protein recognizes a neurite-outgrowth promoting 19-amino acid synthetic peptide (PA 22-2) derived from the laminin A chain. In the present study, an antibody against the 110 kDa LBP was used to localize immunoreactivity in the normal adult rat brain and also following a stab wound and ischemic lesion. Immunoreactive cells were found in layers II/III and V of the cerebral cortex and within apical dendrites of pyramidal neurons. Specific immunoreactivity was also found in the stratum lucidum in the CA3 region of the hippocampus which exhibited densely stained mossy fibers and terminals. Mechanical and ischemic lesions induced intense immunolabeling of reactive glial cells around the lesion site. The distinct and anatomically restricted localization of the immunostain in adult and lesioned rat brain suggests that 110 kDa LBP-like molecules might have an important function in forebrain structures and may be involved in the response to CNS injury.
先前已从小鼠脑中鉴定出一种磷酸化的、分子量约为110 kDa的层粘连蛋白结合蛋白（110 kDa LBP）。这种蛋白能识别一种源自层粘连蛋白A链的促进神经突生长的19个氨基酸的合成肽（PA 22-2）。在本研究中，使用针对110 kDa LBP的抗体在正常成年大鼠脑中以及在刺伤和缺血性损伤后定位免疫反应性。在大脑皮层的II/III层和V层以及锥体神经元的顶端树突内发现了免疫反应性细胞。在海马体CA3区的透明层中也发现了特异性免疫反应性，该区域显示出密集染色的苔藓纤维和终末。机械性和缺血性损伤诱导损伤部位周围反应性胶质细胞的强烈免疫标记。在成年和损伤大鼠脑中免疫染色的独特且解剖学上受限的定位表明，110 kDa LBP样分子可能在前脑结构中具有重要功能，并且可能参与对中枢神经系统损伤的反应。
