Yemets Alla, Stelmakh Oksana, Blume Yaroslav B
Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine.
Cell Biol Int. 2008 Jun;32(6):623-9. doi: 10.1016/j.cellbi.2008.01.012. Epub 2008 Jan 25.
To clarify the mechanism of isopropyl-N-phenyl carbamate (IPC) action on higher plant cells the sensitivity of microtubules (cortical network and mitotic arrays) and microtubule organizing centers to IPC treatment (30 microM) in IPC-resistant and sensitive Nicotiana sylvestris lines was studied. It was clearly demonstrated that IPC does not depolymerize plant MTs but causes the MTOC damage in cells, which results in MTOC fragmentation, splitting of the spindle poles and in abnormal division spindle formation. It was also found that IPC-resistance of mutant N. sylvestris line correlates not with tubulin resistance to IPC action but possibly with resistance of one of the proteins involved in MTOC composition.
为阐明异丙基-N-苯基氨基甲酸酯（IPC）对高等植物细胞的作用机制，研究了抗IPC和敏感的森林烟草品系中微管（皮层网络和有丝分裂阵列）及微管组织中心对IPC处理（30微摩尔）的敏感性。结果清楚表明，IPC不会使植物微管解聚，但会导致细胞中的微管组织中心受损，进而造成微管组织中心碎片化、纺锤极分裂以及异常分裂纺锤体形成。还发现，突变的森林烟草品系对IPC的抗性并非与微管蛋白对IPC作用的抗性相关，而是可能与参与微管组织中心组成的一种蛋白质的抗性有关。
