Sakai K, Nii Y, Ueyama A, Kishino Y
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Cell Mol Biol. 1991;37(3):353-8.
Histochemical localization of cathepsin B in alveolar macrophages (AM) that separated into four different density fractions (I, II, III and IV) by discontinuous Percoll gradient centrifugation was demonstrated in fluorescence microscope using CBZ-Arg-Arg-4-methoxy-2- naphthylamide as a substrate and 5-nitrosalicylaldehyde as a coupling reagent. The least dense AM (fraction I) was found numerous bright yellow fluorescing particles with high intensity in small granules distributed throughout the cytoplasm when compared to the most dense cells (fraction IV). The different localization of cathepsin B activity in the fractionated cells suggested differentiation of lysosomal system and existence of maturational (or aging) sequence in rat AM.
采用CBZ-精氨酸-精氨酸-4-甲氧基-2-萘基酰胺作为底物、5-亚硝基水杨醛作为偶联试剂，在荧光显微镜下证实了通过不连续Percoll梯度离心分离为四个不同密度级分（I、II、III和IV）的肺泡巨噬细胞（AM）中组织蛋白酶B的组织化学定位。与密度最高的细胞（IV级分）相比，密度最低的AM（I级分）在遍布细胞质的小颗粒中发现大量高强度亮黄色荧光颗粒。组织蛋白酶B活性在分级分离细胞中的不同定位表明大鼠AM溶酶体系统的分化以及成熟（或老化）序列的存在。
