Nordström T, Lindqvist C, Ståhls A, Mustelin T, Andersson L C
Department of Pathology, University of Helsinki, Finland.
Cell Calcium. 1991 Jul;12(7):449-55. doi: 10.1016/0143-4160(91)90027-c.
Stimulation of T lymphocytes with antibodies against the T cell receptor/CD3 complex induces within seconds a rise in the concentration of intracellular free Ca2+. Here we show that treatment with 20 microM free myristic acid completely inhibits this Ca2+ signal and the cellular proliferation in Jurkat T cells. Also lauric acid inhibited cell growth while its blocking effect on the Ca2+ signal was weaker than that of myristic acid. Other saturated free fatty acids were inactive. The inhibitory effect of myristic acid could be reversed by the addition of fatty acid free albumin, which will bind the fatty acid. Myristic acid, but not its methyl ester, inhibited both the anti-CD3-induced Ca2+ influx across the cell membrane and Ca2+ release from intracellular stores, but not the formation of inositol phosphates. In contrast, thapsigargin-induced release of Ca2+ from the same intracellular stores was unaffected by myristic acid. Thus, myristic acid specifically blocks T cell antigen receptor-CD3 induced Ca2+ mobilization in T cells.
用抗T细胞受体/CD3复合物的抗体刺激T淋巴细胞会在数秒内导致细胞内游离Ca2+浓度升高。我们在此表明，用20微摩尔游离肉豆蔻酸处理可完全抑制Jurkat T细胞中的这种Ca2+信号和细胞增殖。月桂酸也抑制细胞生长，但其对Ca2+信号的阻断作用比肉豆蔻酸弱。其他饱和游离脂肪酸无活性。肉豆蔻酸的抑制作用可通过添加能结合脂肪酸的无脂肪酸白蛋白来逆转。肉豆蔻酸而非其甲酯，抑制了抗CD3诱导的Ca2+跨细胞膜内流以及细胞内储存库释放Ca2+，但不影响肌醇磷酸的形成。相比之下，毒胡萝卜素诱导的相同细胞内储存库释放Ca2+不受肉豆蔻酸影响。因此，肉豆蔻酸特异性阻断T细胞抗原受体-CD3诱导的T细胞中Ca2+动员。
