Remington Susann G, Crow Jean M, Nelson J Daniel
Department of Ophthalmology, HealthPartners Medical Group and Clinics, St. Paul, Minnesota, USA.
Invest Ophthalmol Vis Sci. 2008 Jul;49(7):2856-62. doi: 10.1167/iovs.07-1496. Epub 2008 Mar 14.
RNAs encoding secretoglobin subunits, also known as lipophilins, are found in rabbit lacrimal glands. Some secretoglobins in other species are tissue- and/or sex-specific, whereas other secretoglobins are expressed in a variety of tissues of both sexes. In this study the tissue-specific and sexual dimorphic expression patterns of rabbit lacrimal gland lipophilins AL, AL2, BL, CL, and CL2 were determined.
RNAs from male and female rabbit lacrimal glands were compared in a differential display analysis, and a new rabbit lacrimal gland secretoglobin, lipophilin AL2, was identified. Next, RNAs from male and female rabbit harderian, lacrimal, mandibular, sublingual, and parotid glands and from liver, kidney, pancreas, testis (male), ovary, and mammary gland (female) were isolated, electrophoresed in agarose gels, and transferred to nylon membranes. cDNA probes encoding the lipophilins AL, AL2, BL, and CL/CL2 were hybridized to the RNA in the blots.
Rabbit mRNAs encoding the lipophilins AL, AL2, BL, CL, and CL2 were detected only in the lacrimal gland. Lipophilin AL2 mRNA was detected only in male rabbit lacrimal gland.
In the rabbit, several lipophilins were expressed only in the lacrimal gland.
编码分泌球蛋白亚基（也称为亲脂素）的RNA在兔泪腺中被发现。其他物种中的一些分泌球蛋白具有组织和/或性别特异性，而其他分泌球蛋白在两性的多种组织中均有表达。在本研究中，确定了兔泪腺亲脂素AL、AL2、BL、CL和CL2的组织特异性和性别二态性表达模式。
在差异显示分析中比较了雄性和雌性兔泪腺的RNA，并鉴定出一种新的兔泪腺分泌球蛋白——亲脂素AL2。接下来，从雄性和雌性兔的哈德氏腺、泪腺、下颌下腺、舌下腺和腮腺以及肝脏、肾脏、胰腺、睾丸（雄性）、卵巢和乳腺（雌性）中分离RNA，在琼脂糖凝胶中进行电泳，然后转移到尼龙膜上。编码亲脂素AL、AL2、BL和CL/CL2的cDNA探针与印迹中的RNA杂交。
编码亲脂素AL、AL2、BL、CL和CL2的兔mRNA仅在泪腺中被检测到。亲脂素AL2 mRNA仅在雄性兔泪腺中被检测到。
在兔中，几种亲脂素仅在泪腺中表达。
