钙离子在组胺能药物对电刺激诱导的大鼠输精管收缩的调节中的作用。

Involvement of calcium ions in the modulation of electrically-induced rat vas deferens contractions by histaminergic drugs.

Vassilev P, Mutafova-Yambolieva V, Staneva-Stoytcheva D, Todorov S

Institute of Physiology, Bulgarian Academy of Sciences, Sofia.

Gen Pharmacol. 1991;22(4):699-702. doi: 10.1016/0306-3623(91)90081-g.

Abstract

The modulatory action of histaminergic drugs: histamine; 2-(2-aminoethyl)thiazole (H1-agonist); dimaprit (H2-agonist); diphenhydramine (H1-receptor antagonist); and cimetidine (H2-receptor antagonist) on rat vas deferens contractions induced by electrical field stimulation (0.1 Hz, 1 pulse, 1 msec duration, supramaximal voltage) was studied either at different calcium concentrations in the nutrient fluid or after verapamil. 2. Histamine and 2-(2-aminoethyl)thiazole inhibited the electrically-evoked contractions (EEC). This effect was unchanged after verapamil. 3. Diphenhydramine potentiated the EEC. Verapamil added before the H1-receptor antagonist inhibited this action. 4. The effects of agonists and antagonists of H-receptors decreased with the increasing of calcium concentration. 5. The results obtained suggested the existence of heterogeneity of prejunctional H-receptors. The function of histaminergic drugs on adrenergic neurotransmission in field stimulated rat vas deferens is Ca(2+)-dependent.

摘要

组胺能药物的调节作用：组胺；2-(2-氨基乙基)噻唑（H1激动剂）；二甲双胍（H2激动剂）；苯海拉明（H1受体拮抗剂）；以及西咪替丁（H2受体拮抗剂）对电场刺激（0.1Hz，1个脉冲，持续时间1毫秒，超最大电压）诱导的大鼠输精管收缩的作用，在营养液中不同钙浓度下或维拉帕米处理后进行了研究。2. 组胺和2-(2-氨基乙基)噻唑抑制电诱发收缩（EEC）。维拉帕米处理后此效应不变。3. 苯海拉明增强EEC。在H1受体拮抗剂之前加入维拉帕米可抑制此作用。4. H受体激动剂和拮抗剂的作用随钙浓度增加而降低。5. 所得结果提示存在节前H受体的异质性。组胺能药物对电场刺激大鼠输精管中肾上腺素能神经传递的作用是钙依赖性的。