Morikawa Y, Kuribayashi K, Yoshikawa F, Fujita K, Mizushima A, Kakudo K
Department of Pathology, Wakayama Medical School, Japan.
Immunology. 1991 Sep;74(1):146-52.
The contribution of B cells and antibodies to the regulation of delayed-type hypersensitivity (DTH) was investigated in mice rendered B-cell-deficient by treatment with anti-mu antibodies. In normal rabbit immunoglobulin (Ig)-treated mice as well as normal mice, the intravenous injection of a large amount of keyhole limpet haemocyanin (KLH) suppressed DTH, and serum titres of the anti-KLH antibody were significantly elevated. However, in anti-mu-treated mice, the intravenous injection of a large amount of KLH could not induce either suppression of DTH or the elevation of anti-KLH antibody titres. The transfer of anti-KLH antibodies suppressed DTH in a H-2 non-restricted, probably Igh-restricted, way in anti-mu-treated mice. In addition, the transfer of anti-KLH antibodies induced effector-phase suppressor T cells whose phenotype was L3T4-, Lyt-2+. We concluded that antibodies play a significant role in the regulation of DTH.
通过用抗μ抗体处理使小鼠B细胞缺陷，研究了B细胞和抗体在迟发型超敏反应（DTH）调节中的作用。在正常兔免疫球蛋白（Ig）处理的小鼠以及正常小鼠中，静脉注射大量钥孔戚血蓝蛋白（KLH）可抑制DTH，并且抗KLH抗体的血清滴度显著升高。然而，在抗μ处理的小鼠中，静脉注射大量KLH既不能诱导DTH的抑制，也不能诱导抗KLH抗体滴度的升高。在抗μ处理的小鼠中，抗KLH抗体的转移以H-2非限制性、可能是Igh限制性的方式抑制DTH。此外，抗KLH抗体的转移诱导了表型为L3T4-、Lyt-2+的效应期抑制性T细胞。我们得出结论，抗体在DTH的调节中起重要作用。
