低代聚丙烯亚胺树枝状大分子接枝β-环糊精：一种用于基因递送系统的高效载体。

Low generation polypropylenimine dendrimer graft beta-cyclodextrin: an efficient vector for gene delivery system.

作者信息

Zhang Wei, Chen Zhe, Song Xiaoxiao, Si Jianmin, Tang Guping

机构信息

Department of Gastroenterology, Institute of Clinical Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou.

出版信息

Technol Cancer Res Treat. 2008 Apr;7(2):103-8. doi: 10.1177/153303460800700202.

DOI:10.1177/153303460800700202

PMID:18345698

Abstract

The purpose of this study was to evaluate the characterization of a new cationic vector. Low generation polypropylenimine (DAB-8) was conjugated to beta-cyclodextrin (beta-CyD), and the chemical characters of the vector were investigated and confirmed by 1H NMR, FT-IR, TGA, TEM, particle size, and zeta potential assay. The biological property was identified by MTT assay and gene transfer efficiency was performed in cell lines. The results showed that the new vector had low cytotoxicity and high transfection efficiency in vitro. This suggested that beta-CyD-DAB-8 has a potential ability to act as a non-viral vector in gene delivery.

摘要

本研究的目的是评估一种新型阳离子载体的特性。低代聚亚丙基亚胺（DAB-8）与β-环糊精（β-CyD）偶联，通过1H NMR、FT-IR、TGA、TEM、粒径和zeta电位测定法对该载体的化学特性进行了研究和确认。通过MTT测定法鉴定生物学特性，并在细胞系中进行基因转移效率检测。结果表明，该新型载体在体外具有低细胞毒性和高转染效率。这表明β-CyD-DAB-8在基因递送中具有作为非病毒载体的潜在能力。