Amorim Leiliane C A, Carneiro Joana P, Cardeal Zenilda L
Department of Chemistry, ICEx, Faculty of Pharmacy, Federal University of Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):141-6. doi: 10.1016/j.jchromb.2008.02.023. Epub 2008 Mar 4.
The determination of benzene in exhaled air has contributed for the increase in the use of breath analysis in biological monitoring. This paper describes SPME as a sampling technique for determining benzene in exhaled air by GC-MS. A system was developed to generate a gaseous benzene standard by a permeation method to accomplish the breath analyses. The method presented good resolution, repeatability (the mean of %RSD values for intra-day measurements was 6.3), sensitivity (2.4 and 3.1 ppb for LOD and LOQ, respectively), and linearity of response (R(2)=0.994). After optimizing the conditions, analyses of real samples were performed on two groups (exposed and not exposed to benzene). The results presented an average of 8.2 ppb for the control group and 25.3 ppb for the exposed group.
呼出气体中苯的测定有助于增加呼吸分析在生物监测中的应用。本文描述了将固相微萃取作为一种通过气相色谱-质谱联用仪测定呼出气体中苯的采样技术。开发了一种通过渗透法生成气态苯标准物质的系统来完成呼吸分析。该方法具有良好的分辨率、重复性（日内测量的相对标准偏差值的平均值为6.3%）、灵敏度（检测限和定量限分别为2.4和3.1 ppb）以及响应线性（R(2)=0.994）。在优化条件后，对两组（接触苯和未接触苯）实际样品进行了分析。结果显示，对照组的平均值为8.2 ppb，接触组为25.3 ppb。
