Okazaki Kaoru, Kondo Masatoshi, Kato Masahiko, Kakinuma Ryota, Nishida Akira, Noda Masahiro, Taniguchi Kiyosu, Kimura Hirokazu
Division of Neonatology, Tokyo Metropolitan Hachioji Children's Hospital, 4-33-13 Daimachi, Hachioji, Tokyo 193-0931, Japan.
Pediatrics. 2008 Apr;121(4):e748-53. doi: 10.1542/peds.2007-1697. Epub 2008 Mar 17.
Various inflammatory cytokines and chemokines are thought to be associated with the pathophysiology of meconium aspiration syndrome. To clarify any such association, we compared various serum cytokine and chemokine profiles in patients with and without meconium aspiration syndrome.
Using a highly sensitive fluorescence microsphere method, 17 types of cytokines and chemokines in sera were measured in 11 neonatal patients with meconium aspiration syndrome, 16 neonatal patients without meconium aspiration syndrome, and 9 healthy children.
The concentrations of 8 types of proinflammatory cytokines and chemokines were significantly higher in the meconium aspiration syndrome group than in healthy controls: interleukin-1beta, interleukin-6, interleukin-8, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, interferon-gamma, macrophage inflammatory protein-1beta, and tumor necrosis factor-alpha. Six types of proinflammatory cytokines and chemokines were significantly higher in the meconium aspiration syndrome group than in the nonmeconium aspiration syndrome group: interleukin-6, interleukin-8, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha. Serum concentrations of interleukin-10 (anti-inflammatory cytokine) in the meconium aspiration syndrome group were higher than those in both the nonmeconium aspiration syndrome group and healthy children group (P = .007 and 0.001, respectively).
Most types of proinflammatory cytokines and chemokines in sera of neonates with meconium aspiration syndrome were higher than those without meconium aspiration syndrome, giving support to the suggestion that elevated levels are associated with the pathogenesis of meconium aspiration syndrome.
多种炎性细胞因子和趋化因子被认为与胎粪吸入综合征的病理生理学相关。为明确此类关联，我们比较了有和没有胎粪吸入综合征患者的各种血清细胞因子和趋化因子谱。
采用高灵敏度荧光微球法，对11例胎粪吸入综合征新生儿患者、16例无胎粪吸入综合征新生儿患者和9名健康儿童的血清中17种细胞因子和趋化因子进行了检测。
胎粪吸入综合征组8种促炎细胞因子和趋化因子的浓度显著高于健康对照组，即白细胞介素-1β、白细胞介素-6、白细胞介素-8、粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子、干扰素-γ、巨噬细胞炎性蛋白-1β和肿瘤坏死因子-α。胎粪吸入综合征组6种促炎细胞因子和趋化因子的浓度显著高于无胎粪吸入综合征组，即白细胞介素-6、白细胞介素-8、粒细胞-巨噬细胞集落刺激因子、粒细胞集落刺激因子、干扰素-γ和肿瘤坏死因子-α。胎粪吸入综合征组白细胞介素-10（抗炎细胞因子）的血清浓度高于无胎粪吸入综合征组和健康儿童组（P值分别为0.007和0.001）。
胎粪吸入综合征新生儿血清中大多数促炎细胞因子和趋化因子的水平高于无胎粪吸入综合征的新生儿，这支持了水平升高与胎粪吸入综合征发病机制相关的观点。
