Sarojini Harshini, Estrada Rosendo, Lu Hongwei, Dekova Silvia, Lee Menq-Jer, Gray Robert D, Wang Eugenia
Gheens Center on Aging, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA.
J Cell Biochem. 2008 Aug 1;104(5):1793-802. doi: 10.1002/jcb.21748.
Conditioned medium (secretome) derived from an enriched stem cell culture stimulates chemotaxis of human fibroblasts. These cells are classified as multipotent murine mesenchymal stromal cells (mMSC) by immunochemical analysis of marker proteins. Proteomic analysis of mMSC secretome identifies nineteen secreted proteins, including extracellular matrix structural proteins, collagen processing enzymes, pigment epithelium-derived factor (PEDF) and cystatin C. Immunodepletion and reconstitution experiments show that PEDF is the predominant fibroblast chemoattractant in the conditioned medium, and immunofluorescence microscopy shows strong staining for PEDF in the cytoplasm, at the cell surface, and in intercellular space between mMSCs. This stimulatory effect of PEDF on fibroblast chemotaxis is in contrast to the PEDF-mediated inhibition of endothelial cell migration, reported previously. These differential functional effects of PEDF toward fibroblasts and endothelial cells may serve to program an ordered temporal sequence of scaffold building followed by angiogenesis during wound healing.
源自富集干细胞培养物的条件培养基（分泌组）可刺激人成纤维细胞的趋化性。通过对标记蛋白的免疫化学分析，这些细胞被分类为多能小鼠间充质基质细胞（mMSC）。对mMSC分泌组的蛋白质组学分析鉴定出19种分泌蛋白，包括细胞外基质结构蛋白、胶原蛋白加工酶、色素上皮衍生因子（PEDF）和胱抑素C。免疫去除和重建实验表明，PEDF是条件培养基中主要的成纤维细胞趋化因子，免疫荧光显微镜显示mMSC的细胞质、细胞表面和细胞间空间中PEDF染色强烈。PEDF对成纤维细胞趋化性的这种刺激作用与先前报道的PEDF介导的内皮细胞迁移抑制作用相反。PEDF对成纤维细胞和内皮细胞的这些不同功能作用可能有助于在伤口愈合过程中编排有序的支架构建时间序列，随后进行血管生成。
