Gregory C Y, Converse C A, Foulds W S
Department of Pharmacy, University of Strathclyde, Glasgow, UK.
Ophthalmic Res. 1991;23(4):171-6. doi: 10.1159/000267100.
A sensitive radioimmunoassay has been developed which allows rapid quantitation of rod outer segment (ROS) phagocytosis by retinal pigment epithelial (RPE) explants in vitro. It involves the use of an antiopsin antiserum, in conjunction with 125I-protein A as a second antibody, and utilizes permeabilization with ethanol to distinguish between the binding and ingestion phases of phagocytosis. This procedure will be used in the future to investigate potential regulatory factors of ROS phagocytosis by retinal pigment epithelium and to evaluate animal models of retinal degeneration.
已开发出一种灵敏的放射免疫测定法，可在体外快速定量视网膜色素上皮（RPE）外植体对杆状体外段（ROS）的吞噬作用。该方法使用抗视蛋白抗血清，并结合125I蛋白A作为二抗，利用乙醇通透化来区分吞噬作用的结合阶段和摄取阶段。该程序将用于未来研究视网膜色素上皮对ROS吞噬作用的潜在调节因子，并评估视网膜变性的动物模型。
