Different conditions for generation of non-cytotoxic autorestricted CD4-CD8+ human suppressor T cells and allospecific cytotoxic T lymphocytes in one-way mixed lymphocyte cultures: the role of adherent mononuclear cells.

The purpose of the present study was to investigate the role of adherent mononuclear cells (AMC) in generation of suppressor T lymphocytes (T-Lph) in one-way allogeneic mixed lymphocyte cultures (MLC). Alloantigen-specific cytotoxic T-Lph were generated in MLC predominantly in the presence of stimulator AMC, but not responder AMC. The presence of responder AMC and the absence of stimulator AMC were optimal conditions for generation of non-cytotoxic (through the whole duration of MLC) suppressor T-Lph. These CD4-CD8+ suppressor T-Lph were allononspecific but autorestricted (with responders in test MLC).