Hübschen Judith M, Kremer Jacques R, De Landtsheer Sébastien, Muller Claude P
Institute of Immunology and WHO Collaborative Centre for Measles and WHO European Regional Reference Laboratory for Measles and Rubella, Laboratoire National de Santé, 20A rue Auguste Lumière, L-1950 Luxembourg, Luxembourg.
J Virol Methods. 2008 May;149(2):246-50. doi: 10.1016/j.jviromet.2008.01.032. Epub 2008 Mar 18.
Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.
麻疹病毒和风疹病毒会引发临床上难以鉴别的发热/出疹性疾病。两种病毒均可在相同的临床标本中检测到，且可在相同的细胞培养物中增殖。本文描述了一种单管多重TaqMan检测法，用于同时快速检测已知基因变异的全谱。该检测法的性能与传统巢式PCR相似，并使用随机引物生成cDNA，可直接用于病毒基因分型。
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