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使用阻抗技术检测冰淇淋中的微生物浓度。

Detection of microbial concentration in ice-cream using the impedance technique.

作者信息

Grossi M, Lanzoni M, Pompei A, Lazzarini R, Matteuzzi D, Riccò B

机构信息

Department of Electronic Engineering , University of Bologna, Via Risorgimento 8, Bologna, Italy.

出版信息

Biosens Bioelectron. 2008 Jun 15;23(11):1616-23. doi: 10.1016/j.bios.2008.01.032. Epub 2008 Feb 12.

Abstract

The detection of microbial concentration, essential for safe and high quality food products, is traditionally made with the plate count technique, that is reliable, but also slow and not easily realized in the automatic form, as required for direct use in industrial machines. To this purpose, the method based on impedance measurements represents an attractive alternative since it can produce results in about 10h, instead of the 24-48h needed by standard plate counts and can be easily realized in automatic form. In this paper such a method has been experimentally studied in the case of ice-cream products. In particular, all main ice-cream compositions of real interest have been considered and no nutrient media has been used to dilute the samples. A measurement set-up has been realized using benchtop instruments for impedance measurements on samples whose bacteria concentration was independently measured by means of standard plate counts. The obtained results clearly indicate that impedance measurement represents a feasible and reliable technique to detect total microbial concentration in ice-cream, suitable to be implemented as an embedded system for industrial machines.

摘要

对于安全和高质量的食品而言,微生物浓度的检测至关重要。传统上,采用平板计数技术进行检测,该技术可靠,但速度慢,且难以按照工业机器直接使用的要求实现自动化。为此,基于阻抗测量的方法是一种有吸引力的替代方案,因为它大约10小时就能得出结果,而标准平板计数需要24 - 48小时,并且可以很容易地实现自动化。本文针对冰淇淋产品对这种方法进行了实验研究。具体而言,考虑了所有真正感兴趣的主要冰淇淋成分,并且未使用营养培养基来稀释样品。利用台式仪器搭建了一个测量装置,用于对样品进行阻抗测量,而样品的细菌浓度则通过标准平板计数法独立测量。所得结果清楚地表明,阻抗测量是检测冰淇淋中总微生物浓度的一种可行且可靠的技术,适合作为工业机器的嵌入式系统来实施。

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