Yang Liju, Bashir Rashid
Biomanufacturing Research Institute and Technology Enterprise (BRITE), Department of Chemistry, North Carolina Central University, Durham, 27707, United States.
Biotechnol Adv. 2008 Mar-Apr;26(2):135-50. doi: 10.1016/j.biotechadv.2007.10.003. Epub 2007 Nov 12.
The realization of rapid, sensitive, and specific methods to detect foodborne pathogenic bacteria is central to implementing effective practice to ensure food safety and security. As a principle of transduction, the impedance technique has been applied in the field of microbiology as a means to detect and/or quantify foodborne pathogenic bacteria. The integration of impedance with biological recognition technology for detection of bacteria has led to the development of impedance biosensors that are finding wide-spread use in the recent years. This paper reviews the progress and applications of impedance microbiology for foodborne pathogenic bacteria detection, particularly the new aspects that have been added to this subject in the past few years, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiology, and the use of equivalent circuits for analysis of the impedance systems. This paper also reviews the significant developments of impedance biosensors for bacteria detection in the past 5 years, focusing on microfabricated microelectrodes-based and microfluidic-based Faradaic electrochemical impedance biosensors, non-Faradaic impedance biosensors, and the integration of impedance biosensors with other techniques such as dielectrophoresis and electropermeabilization.
实现快速、灵敏且特异的食源性病原体检测方法是实施有效措施以确保食品安全与保障的核心。作为一种传感原理，阻抗技术已在微生物学领域中作为检测和/或定量食源性病原体的手段加以应用。将阻抗与用于细菌检测的生物识别技术相结合，已促使阻抗生物传感器得以发展，近年来这类传感器正得到广泛应用。本文综述了阻抗微生物学在食源性病原体检测方面的进展与应用，尤其关注过去几年中该领域新增的内容，包括叉指微电极的使用、基于芯片的阻抗微生物学的发展以及利用等效电路分析阻抗系统。本文还综述了过去5年中用于细菌检测的阻抗生物传感器的重大进展，重点关注基于微纳加工微电极和基于微流控的法拉第电化学阻抗生物传感器、非法拉第阻抗生物传感器，以及阻抗生物传感器与其他技术（如介电电泳和电通透化）的整合。
