一种针对相思子毒素a链的中和抗体在体外和体内均能抑制相思子毒素的毒性。
A neutralizing antibody to the a chain of abrin inhibits abrin toxicity both in vitro and in vivo.
作者信息
Surendranath Kalpana, Karande Anjali A
机构信息
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
出版信息
Clin Vaccine Immunol. 2008 May;15(5):737-43. doi: 10.1128/CVI.00254-07. Epub 2008 Mar 19.
Abstract
Plant ribosome-inactivating proteins (RIPs) are RNA N-glycosidases that inhibit protein synthesis in cells. Abrin, a type II RIP, is an AB type toxin, which is one of the most lethal types of toxin known. The B chain facilitates the entry of the molecule into the cell, whereas the A chain exerts the toxic effect. We have generated hybridomas secreting antibodies of the immunoglobulin G class specific to the recombinant A chain of abrin. One monoclonal antibody, namely, D6F10, rescued cells from abrin toxicity. Importantly, the antibody also protected mice from lethal doses of the toxin. The neutralizing effect of the antibody was shown to be due to interference with abrin attachment to the cell surface.
摘要
植物核糖体失活蛋白（RIPs）是一类RNA N-糖苷酶，可抑制细胞内的蛋白质合成。相思子毒素是一种II型RIP，属于AB型毒素，是已知毒性最强的毒素之一。B链促进该分子进入细胞，而A链发挥毒性作用。我们已制备出分泌针对重组相思子毒素A链的免疫球蛋白G类抗体的杂交瘤。一种单克隆抗体，即D6F10，可使细胞免受相思子毒素的毒性作用。重要的是，该抗体还能保护小鼠免受致死剂量毒素的侵害。抗体的中和作用被证明是由于干扰了相思子毒素与细胞表面的结合。
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本文引用的文献
1
Evidence for widespread epithelial damage and coincident production of monocyte chemotactic protein 1 in a murine model of intestinal ricin intoxication.
Infect Immun. 2007 Apr;75(4):1745-50. doi: 10.1128/IAI.01528-06. Epub 2007 Feb 5.
2
Structure-function analysis and insights into the reduced toxicity of Abrus precatorius agglutinin I in relation to abrin.
J Biol Chem. 2006 Nov 10;281(45):34465-74. doi: 10.1074/jbc.M601777200. Epub 2006 Jun 13.
3
Characterization of a novel high-affinity monoclonal immunoglobulin G antibody against the ricin B subunit.
Infect Immun. 2006 Jun;74(6):3463-70. doi: 10.1128/IAI.00324-06.
4
Immunoglobulin A antibodies against ricin A and B subunits protect epithelial cells from ricin intoxication.
Infect Immun. 2006 Jun;74(6):3455-62. doi: 10.1128/IAI.02088-05.
5
A pilot clinical trial of a recombinant ricin vaccine in normal humans.
Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2268-73. doi: 10.1073/pnas.0510893103. Epub 2006 Feb 3.
6
Occupational sensitization to ribosome-inactivating proteins in researchers.
Clin Exp Allergy. 2005 Oct;35(10):1354-60. doi: 10.1111/j.1365-2222.2005.02338.x.
7
The evolving field of biodefence: therapeutic developments and diagnostics.
Nat Rev Drug Discov. 2005 Apr;4(4):281-97. doi: 10.1038/nrd1694.
8
Ribosome inactivating proteins and apoptosis.
FEBS Lett. 2005 Feb 28;579(6):1324-31. doi: 10.1016/j.febslet.2005.01.038.
9
Antibody therapy in the management of shiga toxin-induced hemolytic uremic syndrome.
Clin Microbiol Rev. 2004 Oct;17(4):926-41, table of contents. doi: 10.1128/CMR.17.4.926-941.2004.
10
Antitoxins: novel strategies to target agents of bioterrorism.
Nat Rev Microbiol. 2004 Sep;2(9):721-6. doi: 10.1038/nrmicro977.
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