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Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.通过细胞核的荧光激活分选对细胞特异性基因表达进行全局表征。
Plant Physiol. 2008 May;147(1):30-40. doi: 10.1104/pp.107.115246. Epub 2008 Mar 19.
2
Flow cytometry and sorting in Arabidopsis.拟南芥中的流式细胞术与分选
Methods Mol Biol. 2014;1062:509-37. doi: 10.1007/978-1-62703-580-4_27.
3
Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice.用于分析拟南芥和水稻中细胞类型对盐胁迫特异性反应的荧光激活细胞分选技术。
Methods Mol Biol. 2012;913:265-76. doi: 10.1007/978-1-61779-986-0_18.
4
Multiparametric analysis, sorting, and transcriptional profiling of plant protoplasts and nuclei according to cell type.根据细胞类型对植物原生质体和细胞核进行多参数分析、分选及转录谱分析。
Methods Mol Biol. 2011;699:407-29. doi: 10.1007/978-1-61737-950-5_20.
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Arabidopsis HIT4 encodes a novel chromocentre-localized protein involved in the heat reactivation of transcriptionally silent loci and is essential for heat tolerance in plants.拟南芥 HIT4 编码一种新型的着丝粒定位蛋白，参与转录沉默基因座的热激活，对于植物的耐热性至关重要。
J Exp Bot. 2013 Apr;64(6):1689-701. doi: 10.1093/jxb/ert030. Epub 2013 Feb 13.
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A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance.一种真菌转录因子基因由其自身启动子在植物中表达，并提高耐旱性。
Planta. 2015 Jul;242(1):39-52. doi: 10.1007/s00425-015-2285-5. Epub 2015 Mar 26.
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Arabidopsis P-protein filament formation requires both AtSEOR1 and AtSEOR2.拟南芥 P 蛋白丝的形成需要 AtSEOR1 和 AtSEOR2。
Plant Cell Physiol. 2012 Jun;53(6):1033-42. doi: 10.1093/pcp/pcs046. Epub 2012 Apr 1.
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Tissue-specific transcriptome profiling of the Arabidopsis inflorescence stem reveals local cellular signatures.拟南芥花序茎组织特异性转录组分析揭示局部细胞特征。
Plant Cell. 2021 Apr 17;33(2):200-223. doi: 10.1093/plcell/koaa019.
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Cloning and characterization of a novel Athspr promoter specifically active in vascular tissue.一种在维管组织中特异性活跃的新型拟南芥启动子的克隆与特性分析。
Plant Physiol Biochem. 2014 May;78:88-96. doi: 10.1016/j.plaphy.2014.02.019. Epub 2014 Mar 3.
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Flow Cytometry and Sorting in Arabidopsis.拟南芥的流式细胞术与分选
Methods Mol Biol. 2021;2200:255-294. doi: 10.1007/978-1-0716-0880-7_12.
引用本文的文献
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Comparison of transcriptional activity profiling by metabolic labeling or nuclear RNA sequencing.通过代谢标记或核RNA测序进行转录活性谱分析的比较。
Plant J. 2025 Aug;123(3):e70401. doi: 10.1111/tpj.70401.
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Single-Nucleus Transcriptomics Reveals How Cell Type Shapes the Circadian Transcriptome of the Arabidopsis Leaf.单核转录组学揭示细胞类型如何塑造拟南芥叶片的昼夜转录组。
bioRxiv. 2025 Jul 16:2025.06.12.659411. doi: 10.1101/2025.06.12.659411.
3
Tomato HAIRY MERISTEM4, expressed in the phloem, is required for proper shoot and fruit development.在韧皮部表达的番茄毛状分生组织4对茎和果实的正常发育是必需的。
Hortic Res. 2024 Nov 21;12(3):uhae325. doi: 10.1093/hr/uhae325. eCollection 2025 Mar.
4
Fluorescence-activated nuclear sorting (FANS) of nuclei from in vitro-generated syncytiotrophoblast.对体外生成的合体滋养层细胞核进行荧光激活核分选（FANS）。
Placenta. 2024 Jun 13. doi: 10.1016/j.placenta.2024.06.007.
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Loss of linker histone H1 in the maternal genome influences DEMETER-mediated demethylation and affects the endosperm DNA methylation landscape.母本基因组中连接组蛋白H1的缺失影响了由DEMETER介导的去甲基化作用，并影响胚乳DNA甲基化格局。
Front Plant Sci. 2022 Dec 22;13:1070397. doi: 10.3389/fpls.2022.1070397. eCollection 2022.
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A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells.根韧皮部柱细胞图谱揭示了与原韧皮部相邻细胞中的常见转录状态。
Nat Plants. 2022 Aug;8(8):954-970. doi: 10.1038/s41477-022-01178-y. Epub 2022 Aug 4.
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Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato.利用gwINTACT技术解析番茄中精确的基因转录表达
Front Plant Sci. 2022 Apr 14;13:852206. doi: 10.3389/fpls.2022.852206. eCollection 2022.
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The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata.玉米单核转录组全面描述了调控草类气孔运动和发育的信号网络。
Plant Cell. 2022 Apr 26;34(5):1890-1911. doi: 10.1093/plcell/koac047.
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Auxin Metabolite Profiling in Isolated and Intact Plant Nuclei.在分离和完整的植物细胞核中进行生长素代谢产物分析。
Int J Mol Sci. 2021 Nov 16;22(22):12369. doi: 10.3390/ijms222212369.
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OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security.用于粮食和营养安全的组学、表观遗传学及基因组编辑技术
Plants (Basel). 2021 Jul 12;10(7):1423. doi: 10.3390/plants10071423.
本文引用的文献
1
Apical organization and maturation of the cortex and vascular cylinder inArabidopsis thaliana (Brassicaceae) roots.拟南芥（十字花科）根中皮层和维管束的顶端组织和成熟。
Am J Bot. 2002 Jun;89(6):908-20. doi: 10.3732/ajb.89.6.908.
2
A high-resolution root spatiotemporal map reveals dominant expression patterns.高分辨率根系时空图谱揭示了主要表达模式。
Science. 2007 Nov 2;318(5851):801-6. doi: 10.1126/science.1146265.
3
Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells.人类细胞中细胞核和细胞质区室对整体基因表达贡献的比较。
BMC Genomics. 2007 Sep 25;8:340. doi: 10.1186/1471-2164-8-340.
4
Functional sieve element protoplasts.功能性筛管分子原生质体
Plant Physiol. 2007 Nov;145(3):703-11. doi: 10.1104/pp.107.105940. Epub 2007 Sep 20.
5
Rapid flow cytometric analysis of the cell cycle in intact plant tissues.快速流式细胞术分析完整植物组织中的细胞周期。
Science. 1983 Jun 3;220(4601):1049-51. doi: 10.1126/science.220.4601.1049.
6
A rice dihydrosphingosine C4 hydroxylase (DSH1) gene, which is abundantly expressed in the stigmas, vascular cells and apical meristem, may be involved in fertility.一种在柱头、维管细胞和顶端分生组织中大量表达的水稻二氢鞘氨醇C4羟化酶（DSH1）基因可能与育性有关。
Plant Cell Physiol. 2007 Aug;48(8):1108-20. doi: 10.1093/pcp/pcm084. Epub 2007 Jul 2.
7
RNA-binding proteins: modular design for efficient function.RNA结合蛋白：高效功能的模块化设计
Nat Rev Mol Cell Biol. 2007 Jun;8(6):479-90. doi: 10.1038/nrm2178.
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Deficiency in phosphatidylserine decarboxylase activity in the psd1 psd2 psd3 triple mutant of Arabidopsis affects phosphatidylethanolamine accumulation in mitochondria.拟南芥psd1 psd2 psd3三重突变体中磷脂酰丝氨酸脱羧酶活性的缺陷影响线粒体中磷脂酰乙醇胺的积累。
Plant Physiol. 2007 Jun;144(2):904-14. doi: 10.1104/pp.107.095414. Epub 2007 Apr 20.
9
Sphingolipids and cell death.鞘脂与细胞死亡。
Apoptosis. 2007 May;12(5):923-39. doi: 10.1007/s10495-007-0721-0.
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Global studies of cell type-specific gene expression in plants.植物中细胞类型特异性基因表达的全球研究。
Annu Rev Plant Biol. 2006;57:451-75. doi: 10.1146/annurev.arplant.57.032905.105302.
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