Peressutti S R, Olivera N L, Babay P A, Costagliola M, Alvarez H M
Instituto Nacional de Investigación y Desarrollo Pesquero, Mar del Plata, Argentina.
J Appl Microbiol. 2008 Aug;105(2):476-84. doi: 10.1111/j.1365-2672.2008.03771.x. Epub 2008 Mar 17.
To isolate and identify linear alkylbenzene sulfonate (LAS)-degrading bacteria from Río de la Plata and adjacent waters, and to assay their degradation capability as a consortium and as single organisms.
A consortium consisting of four bacterial strains: Aeromonas caviae (two strains), Pseudomonas alcaliphila and Vibrio sp. was identified by 16S rRNA analysis. Isolates grown as a consortium produced higher biomass from LAS and CO(2) release (mineralization) than individual cultures, and degraded 86% of LAS (20 mg l(-1)), whereas pure strains degraded between 21% and 60%. Bacterial desulfonation from LAS was evidenced in the consortium and A. caviae strains. A complete disappearance of LAS (10 mg l(-1)) was accomplished, and LAS levels of 50 and 100 mg l(-1) led to a pronounced decrease in the biodegradation extent and inhibition of culture growth.
A bacterial consortium capable of complete LAS degradation was isolated from the Río de la Plata and adjacent waters. This consortium was more efficient for LAS degradation than individual cultures, and was sensitive to high LAS concentrations.
The autochthonous consortium with high effectiveness on LAS biodegradation is a useful tool for LAS depletion from these polluted ecosystems.
从拉普拉塔河及邻近水域分离并鉴定能降解直链烷基苯磺酸盐（LAS）的细菌，并检测它们作为混合菌群和单一菌株时的降解能力。
通过16S rRNA分析鉴定出一个由四株细菌组成的混合菌群，包括豚鼠气单胞菌（两株）、嗜碱假单胞菌和弧菌属。作为混合菌群生长的分离物从LAS产生的生物量和二氧化碳释放量（矿化）高于单独培养物，能降解86%的LAS（20毫克/升），而纯菌株的降解率在21%至60%之间。在混合菌群和豚鼠气单胞菌菌株中证实了LAS的细菌脱硫作用。实现了LAS（10毫克/升）的完全消失，50和100毫克/升的LAS水平导致生物降解程度显著降低并抑制培养物生长。
从拉普拉塔河及邻近水域分离出了一个能够完全降解LAS的细菌混合菌群。该混合菌群对LAS的降解比单独培养物更有效，并且对高LAS浓度敏感。
对LAS生物降解具有高效性的本地混合菌群是从这些受污染生态系统中去除LAS的有用工具。
