Izumi Hironari, Cairney John W G, Killham Ken, Moore Edward, Alexander Ian J, Anderson Ian C
The Macaulay Institute, Craigiebuckler, Aberdeen, Scotland, UK.
FEMS Microbiol Lett. 2008 May;282(2):196-204. doi: 10.1111/j.1574-6968.2008.01122.x. Epub 2008 Mar 18.
Bacterial communities associated with ectomycorrhizal and uncolonized roots of Pinus elliottii (slash pine) collected from a plantation in south-east Queensland, Australia, were investigated, using cultivation-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene PCR products obtained using a cultivation-independent approach revealed that bacterial communities associated with ectomycorrhizal root tips differed significantly from those associated with roots uncolonized by ectomycorrhizal fungi. DGGE analysis of cultivable bacterial communities revealed no significant difference between ectomycorrhizal and uncolonized roots. Neither analytical approach revealed significant differences between the bacterial communities associated with ectomycorrhizal roots colonized by a Suillus sp. or an Atheliaceae taxon. Cloned bacterial 16S rRNA genes revealed sequence types closely related with that of Burkholderia phenazinium, common in both ectomycorrhizal-colonized and -uncolonized roots, while sequence types most similar to the potentially phyopathogenic bacteria Burkholderia andropogonis and Pantoea ananatis were only detected in ectomycorrhizal roots. These results highlight the possibility of global movement of microorganisms, including putative pathogens, as a result of the introduction of exotic pine plantations.
利用依赖培养和不依赖培养的方法，对从澳大利亚昆士兰州东南部一个种植园采集的湿地松外生菌根和未被定殖的根系相关细菌群落进行了研究。使用不依赖培养方法获得的16S rRNA基因PCR产物的变性梯度凝胶电泳（DGGE）分析表明，与外生菌根根尖相关的细菌群落与未被外生菌根真菌定殖的根系相关细菌群落有显著差异。可培养细菌群落的DGGE分析表明，外生菌根根系和未被定殖的根系之间没有显著差异。两种分析方法均未揭示与被滑锈伞属或阿太菌科分类单元定殖的外生菌根根系相关的细菌群落之间存在显著差异。克隆的细菌16S rRNA基因显示，序列类型与在被外生菌根定殖和未被定殖的根系中均常见的吩嗪伯克霍尔德菌密切相关，而与潜在致病性细菌安德罗波戈尼斯伯克霍尔德菌和菠萝泛菌最相似的序列类型仅在外生菌根根系中检测到。这些结果突出了由于引入外来松树种植园，包括假定病原体在内的微生物全球传播的可能性。
