Mendes Luiz F, Bastos Erick L, Desjardin Dennis E, Stevani Cassius V
Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
FEMS Microbiol Lett. 2008 May;282(1):132-9. doi: 10.1111/j.1574-6968.2008.01118.x. Epub 2008 Mar 18.
Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova. Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidity show optimal bioluminescence emission at 25 degrees C in the presence of 1.0% sugar cane molasses, 0.10% yeast extract and pH 6.0 (nonbuffered). Temperature and pH are the most important factors for both mycelial growth and bioluminescence.
