Radwan-Oczko Małgorzata, Boratyńska Maria, Zietek Marek, Dobosz Tadeusz
Department of Periodontology, Silesian Piasts University of Medicine, Wrocław, Poland.
J Clin Periodontol. 2008 May;35(5):371-8. doi: 10.1111/j.1600-051X.2008.01216.x. Epub 2008 Mar 17.
The relationship between gingival overgrowth (GO) induced by cyclosporine A (CsA) and transforming growth factor-beta1 (TGF-beta1) remains unclear. The aims of the present study were to evaluate TGF-beta1 gene expression under different immunosuppressive treatments and its association with TGF-beta1 gene functional polymorphism and GO in renal transplant recipients.
The study included 98 CsA-treated renal transplant recipients (with and without GO) and 44 tacrolimus-treated transplant patients (without GO). TGF-beta1 mRNA expression was measured using a real-time quantitative polymerase chain reaction assay. The levels were correlated with TGF-beta1 gene polymorphisms at codons 10 and 25, with different immunosuppressive treatment and GO.
The level of TGF-beta1 gene expression was insignificantly lower in the CsA-treated group compared with the tacrolimus group, and significantly lower in the group with GO compared with patients without GO. In tacrolimus- and CsA-treated patients, but not in patients with GO, the level of TGF-beta1 gene expression was associated with functional phenotypes of TGF-beta1. The incidence, degree and extent of GO were higher in recipients with lower TGF-beta1 gene expression.
Lower level TGF-beta1 gene expression, not functional polymorphism, in patients treated with CsA may be considered to be a risk factor for GO.
环孢素A（CsA）诱导的牙龈过度生长（GO）与转化生长因子-β1（TGF-β1）之间的关系尚不清楚。本研究的目的是评估不同免疫抑制治疗下TGF-β1基因表达及其与TGF-β1基因功能多态性和肾移植受者GO的关联。
本研究纳入98例接受CsA治疗的肾移植受者（有或无GO）和44例接受他克莫司治疗的移植患者（无GO）。采用实时定量聚合酶链反应法检测TGF-β1 mRNA表达。这些水平与第10和25密码子处的TGF-β1基因多态性、不同的免疫抑制治疗及GO相关。
与他克莫司组相比，CsA治疗组TGF-β1基因表达水平无显著降低，与无GO的患者相比，有GO的组中该水平显著降低。在接受他克莫司和CsA治疗的患者中，但在有GO的患者中并非如此，TGF-β1基因表达水平与TGF-β1的功能表型相关。TGF-β1基因表达较低的受者中GO的发生率、程度和范围更高。
CsA治疗患者中较低水平的TGF-β1基因表达而非功能多态性可能被认为是GO的一个危险因素。
