Yuan Jipei, Guo Weiwei, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Biosens Bioelectron. 2008 May 15;23(10):1567-71. doi: 10.1016/j.bios.2008.01.008. Epub 2008 Jan 17.
In this paper, we attempt to develop a sensitive detection method for glucose with the combination of the unique optical property of quantum dots and the specificity of enzymatic reactions. With glucose and hydroquinone as substrates, benzoquinone that intensively quenches the photoluminescence of quantum dots can be produced via the catalysis of bienzyme (glucose oxidase and horseradish peroxidase) system. A relatively low detection limit of 1.0x10(-8)mol/L can be achieved. Two linear ranges from 1.0x10(-6) to 1.5x10(-4)M and from 1.5x10(-4) to 1.0x10(-3)M were obtained. For the detection of 1.0x10(-4)M glucose, six replicative measurements showed the reproducibility (R.S.D.) of 4.43%. The quantum dots-enzymes system possesses advantages of simple procedure without modification of quantum dots or immobilization of enzymes, low cost, high sensitivity and short detection times within several minutes.
在本文中，我们尝试结合量子点独特的光学性质和酶促反应的特异性，开发一种用于葡萄糖的灵敏检测方法。以葡萄糖和对苯二酚为底物，通过双酶（葡萄糖氧化酶和辣根过氧化物酶）体系的催化作用，可以生成能强烈猝灭量子点光致发光的苯醌。可实现相对较低的检测限，为1.0×10⁻⁸mol/L。获得了两个线性范围，分别为1.0×10⁻⁶至1.5×10⁻⁴M以及1.5×10⁻⁴至1.0×10⁻³M。对于1.0×10⁻⁴M葡萄糖的检测，六次重复测量显示重现性（相对标准偏差）为4.43%。量子点 - 酶体系具有操作步骤简单、无需对量子点进行修饰或固定化酶、成本低、灵敏度高以及在几分钟内检测时间短等优点。
