Kato Y, Morisawa T, Kuramoto H
Department of Obstetrics and Gynecology, School of Medicine, Kitasato University.
Hum Cell. 1991 Jun;4(2):165-70.
By using ovariectomized nude mice, the hormone reactivity of endometrial carcinoma was evaluated. HEC-88nu cultured cells originated from human endometrial carcinoma were first transplanted to the animal in each experiment. Estrogen receptor (ER) of HEC-88nu reveals positive originally. Hormone pellets containing medroxyprogesterone acetate (MPA) and 17 beta-estradiol (E2) were used. The results were as follows: 1. The proliferation of this tumor was accelerated remarkably by administration of E2 pellet. 2. By administration of MPA pellet, the proliferation was inhibited from the beginning but progressed flatly afterwards maintaining 50% of the control. 3. When MPA was administered upon priming the tumor with E2, the proliferation began to be inhibited after 2 weeks developing 60% of suppression 5 weeks later. 4. Progesterone receptor (PR) of the tumor was induced starting at week 2 when E2 was given and revealed 189 fmol/mgP at week 5. 5. As the morphological changes due to hormone, light eosin-stainability, rarefaction and swelling of the cytoplasms were the common characteristics. 6. It was suggested that both hormonal and pharmacological actions take part in the mechanism of progestin to act on endometrial carcinoma.
通过使用去卵巢裸鼠，评估子宫内膜癌的激素反应性。在每个实验中，首先将源自人子宫内膜癌的HEC - 88nu培养细胞移植到动物体内。HEC - 88nu的雌激素受体（ER）最初显示为阳性。使用含有醋酸甲羟孕酮（MPA）和17β - 雌二醇（E2）的激素药丸。结果如下：1. 给予E2药丸可显著加速该肿瘤的增殖。2. 给予MPA药丸后，增殖从一开始就受到抑制，但之后平缓进展，维持在对照的50%。3. 在用E2预处理肿瘤后给予MPA，增殖在2周后开始受到抑制，5周后抑制率达60%。4. 当给予E2时，肿瘤的孕激素受体（PR）在第2周开始诱导，在第5周时显示为189 fmol/mgP。5. 作为激素引起的形态学变化，细胞质轻度嗜酸性染色、稀疏和肿胀是共同特征。6. 提示激素和药理作用均参与孕激素作用于子宫内膜癌的机制。
