Sargent D J, Cipriani G, Vilanova S, Gil-Ariza D, Arús P, Simpson D W, Tobutt K R, Monfort A
East Malling Research, New Road, East Malling, Kent ME19 6BJ, UK.
Genome. 2008 Feb;51(2):120-7. doi: 10.1139/g07-107.
We have identified a set of plants (the bin set) to permit "selective" or "bin" mapping using the diploid strawberry mapping population FV x FN, derived from the F2 cross F. vesca 815 x F. nubicola 601, which has been used to develop the Fragaria reference map. The bin set consists of 8 plants: the F. vesca 815 parent, the F1 hybrid individual, and 6 seedlings of the F2 population. This bin set divides the 578 cM of the diploid Fragaria genome into 46 bins, the largest mapping bin being 26 cM in length and the average bin size being 12.6 cM. To validate the FV x FN bin set, we used it to locate 103 loci into bins on the FV x FN map. These loci comprised 61 previously described SSRs, 38 new SSRs developed in this investigation from Fragaria x ananassa genomic DNA, EST and gene sequences, and 4 ripening-related genes developed for Prunus. The 103 markers were located to bins on all 7 linkage groups of the Fragaria map and a new mapping bin was identified with the novel markers, demonstrating that the map covers the majority of the diploid Fragaria genome and that the 6 bin-set seedlings selected were appropriate for bin mapping using this progeny.
我们已鉴定出一组植物（bin集），以便使用源自F2杂交种野草莓815×云雾草莓601的二倍体草莓作图群体FV×FN进行“选择性”或“bin”作图，该群体已用于构建草莓参考图谱。bin集由8株植物组成：野草莓815亲本、F1杂交个体以及F2群体的6株幼苗。这个bin集将二倍体草莓基因组的578厘摩（cM）划分为46个bin，最大的作图bin长度为26 cM，平均bin大小为12.6 cM。为了验证FV×FN bin集，我们用它将103个基因座定位到FV×FN图谱的bin中。这些基因座包括61个先前描述的简单序列重复（SSR）、本研究中从凤梨草莓基因组DNA、表达序列标签（EST）和基因序列开发的38个新SSR，以及为李属开发的4个与成熟相关的基因。这103个标记被定位到草莓图谱所有7个连锁群的bin中，并且用新标记鉴定出一个新的作图bin，表明该图谱覆盖了二倍体草莓基因组的大部分，并且所选的6株bin集幼苗适用于使用该后代进行bin作图。
