Williams N E, Macey M G
Department of Biology, University of Iowa, Iowa City 52242.
Exp Cell Res. 1991 Dec;197(2):137-9. doi: 10.1016/0014-4827(91)90414-p.
Biochemical evidence is presented for the presence of cyclin in Tetrahymena. Zeuthen previously postulated the existence of a heat-labile "division protein" to explain heat-shock-induced division synchrony in Tetrahymena [(1964) Synchrony in Cell Division and Growth (Zeuthen, E., Ed.), pp. 99-158, Interscience, New York]. We show that cyclin is heat-labile in Tetrahymena and suggest that cyclin may be Zeuthen's division protein. Cyclin and cell cycle control is of interest in Tetrahymena because the division mechanism drives macronuclear amitosis, closed and acentric micronuclear mitosis, and cortical differentiation in this cell type.
本文提供了四膜虫中存在细胞周期蛋白的生化证据。泽滕此前推测存在一种热不稳定的“分裂蛋白”，以解释热休克诱导的四膜虫分裂同步性[（1964年）《细胞分裂与生长中的同步性》（泽滕，E.编），第99 - 158页，跨学科出版社，纽约]。我们发现细胞周期蛋白在四膜虫中是热不稳定的，并提出细胞周期蛋白可能就是泽滕所说的分裂蛋白。细胞周期蛋白和细胞周期调控在四膜虫中备受关注，因为这种细胞类型的分裂机制驱动着大核无丝分裂、封闭且无中心粒的小核有丝分裂以及皮层分化。
