Hong Ran, Lim Sung-Chul
Department of Pathology, College of Medicine, Chosun University, Gwangju 501-140, Korea.
Oncol Rep. 2008 Apr;19(4):913-9.
This study was conducted to determine the level of expression and cellular localization of connexin 26 (Cx26) and the expression of p53 in colorectal adenocarcinoma as well as their relationship to clinicopathological features. Immunohistochemical staining was performed in 130 colorectal adenocarcinoma cases. A correlation between the expression levels of the two proteins and an analysis of the clinicopathological features of the samples was performed. There was a statistical significant difference in the Cx26 expression level among normal epithelium (NE), adenomas and adenocarcinomas (p<0.001). Of the 130 adenocarcinomas, 48.5% were positive for Cx26. All of the adenoma and NE samples were positive for Cx26 expression; however, the level of expression of Cx26 in adenomas was smaller than the level of expression for NE. Cytoplasmic staining for Cx26 was observed in the adenocarcinomas (23.8%), but was not observed in the adenoma and NE samples. A positive correlation between a reduction in intercellular Cx26 and tumor invasion was statistically significant (p<0.05). Expression of p53 was positive for 50% of the adenocarcinomas, and the level of p53 was increased in a reverse proportion to the level of Cx26 intercellular staining. In conclusion, loss of intercellular and gain of intracytoplasmic Cx26 expression may play a role in colorectal carcinogenesis and tumor progression.
本研究旨在确定结直肠癌中连接蛋白26（Cx26）的表达水平、细胞定位以及p53的表达情况，及其与临床病理特征的关系。对130例结直肠癌病例进行了免疫组织化学染色。对两种蛋白的表达水平进行相关性分析，并对样本的临床病理特征进行分析。正常上皮（NE）、腺瘤和腺癌之间Cx26表达水平存在统计学显著差异（p<0.001）。在130例腺癌中，48.5%的Cx26呈阳性。所有腺瘤和NE样本的Cx26表达均为阳性；然而，腺瘤中Cx26的表达水平低于NE。在腺癌中观察到Cx26的细胞质染色（23.8%），但在腺瘤和NE样本中未观察到。细胞间Cx26减少与肿瘤侵袭之间的正相关具有统计学意义（p<0.05）。50%的腺癌p53表达呈阳性，且p53水平与细胞间Cx26染色水平呈反比增加。总之，细胞间Cx26的缺失和细胞质内Cx26表达的增加可能在结直肠癌发生和肿瘤进展中起作用。
