Volynets' H P, Trykash I O
Ukr Biokhim Zh (1999). 2007 Sep-Oct;79(5):98-108.
Fusion of synaptic vesicles with various target membranes was investigated on the cell-free model system that reflects the final step of exocytosis. Plasma membranes, synaptic vesicles and liposomes were used as acceptor membranes. The process of membrane fusion was triggered by Ca2+. We have demonstrated that synaptic vesicles are prone to fuse with liposomes in buffer solution. This process was strongly dependent on ionic force of medium and phospholipid composition of liposomes. Cytosolic proteins of synaptosomes inhibited the fusion of synaptic vesicles with liposomes, while these were required for the fusion of synaptic vesicles with native membrane structures. Trypsinolysis of acceptor membranes markedly inhibited the fusion response. It means protein components of target membrane are necessary for realization of the final step of exocytosis.
在反映胞吐作用最后一步的无细胞模型系统上，研究了突触小泡与各种靶膜的融合。质膜、突触小泡和脂质体用作受体膜。膜融合过程由Ca2+触发。我们已经证明，突触小泡在缓冲溶液中易于与脂质体融合。这个过程强烈依赖于介质的离子强度和脂质体的磷脂组成。突触体的胞质蛋白抑制突触小泡与脂质体的融合，而这些蛋白是突触小泡与天然膜结构融合所必需的。受体膜的胰蛋白酶消化显著抑制融合反应。这意味着靶膜的蛋白质成分对于实现胞吐作用的最后一步是必要的。
